ArticleAMB Express2017
Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli.
Article in AMB Express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Adaptive Laboratory Evolution and Carbon/Nitrogen Imbalance Promote High-Yield Ammonia Release inMicroorganisms · 2025Article
- A synthetic microbial Daisyworld: planetary regulation in the test tube.Journal of the Royal Society, Interface · 2024Article
- Developing rumen mimicry process for biological ammonia synthesis.Bioprocess and biosystems engineering · 2023Article
- Trends in Biological Ammonia Production.Biotech (Basel (Switzerland)) · 2023Review
- Amino Acid Availability Determines Plant Immune Homeostasis in the Rhizosphere Microbiome.mBio · 2023Article
- Ammonia Production Using Bacteria and Yeast toward a Sustainable Society.Bioengineering (Basel, Switzerland) · 2023Review
- Fresh Crab Plays an Important Role as a Nutrient Reservoir for the Rapid Propagation ofFrontiers in microbiology · 2021Article
- Efficient ammonia production from food by-products by engineered Escherichia coli.AMB Express · 2020Article
- Wax ester production in nitrogen-rich conditions by metabolically engineeredMetabolic engineering communications · 2020Article
- Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara).AMB Express · 2020Article
- Biorefining of protein waste for production of sustainable fuels and chemicals.Biotechnology for biofuels · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The demand for ammonia is expected to increase in the future because of its importance in agriculture, industry, and hydrogen transportation. Although the Haber-Bosch process is known as an effective way to produce ammonia, the process is energy-intensive. Thus, an environmentally friendly ammonia production process is desired. In this study, we aimed to produce ammonia from amino acids and amino acid-based biomass-like resources by modifying the metabolism of Escherichia coli. By engineering metabolic flux to promote ammonia production using the overexpression of the ketoisovalerate decarboxylase gene (kivd), derived from Lactococcus lactis, ammonia production from amino acids was 351 mg/L (36.6% yield). Furthermore, we deleted the glnA gene, responsible for ammonia assimilation. Using yeast extract as the sole source of carbon and nitrogen, the resultant strain produced 458 mg/L of ammonia (47.8% yield) from an amino acid-based biomass-like material. The ammonia production yields obtained are the highest reported to date. This study suggests that it will be possible to produce ammonia from waste biomass in an environmentally friendly process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.