ArticleApplied microbiology and biotechnology2017
Characterisation of three fungal glucuronoyl esterases on glucuronic acid ester model compounds.
Article in Applied microbiology and biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 38 citations in OpenAlex.
- Glucuronoyl Esterase Expressed in Aspen Xylem Affects γ-Ester Linkages Between Lignin and Glucuronoxylan Reducing Recalcitrance and Accelerating Growth.Plant biotechnology journal · 2025Article
- Modifying lignin composition and xylan O-acetylation induces changes in cell wall composition, extractability, and digestibility.Biotechnology for biofuels and bioproducts · 2024Article
- Exploration of three Dyadobacter fermentans enzymes uncovers molecular activity determinants in CE15.Applied microbiology and biotechnology · 2024Article
- Polysaccharide utilization loci from Bacteroidota encode CE15 enzymes with possible roles in cleaving pectin-lignin bonds.Applied and environmental microbiology · 2024Article
- Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans.Acta crystallographica. Section D, Structural biology · 2023Article
- Glucuronoyl esterases - enzymes to decouple lignin and carbohydrates and enable better utilization of renewable plant biomass.Essays in biochemistry · 2023Review
- Generation of a Library of Carbohydrate-Active Enzymes for Plant Biomass Deconstruction.International journal of molecular sciences · 2022Article
- Mechanism and biomass association of glucuronoyl esterase: an α/β hydrolase with potential in biomass conversion.Nature communications · 2022Article
- Structural and Functional Analysis of a Multimodular Hyperthermostable Xylanase-Glucuronoyl Esterase fromBiochemistry · 2021Article
- Ascertaining the biochemical function of an essential pectin methylesterase in the gut microbeThe Journal of biological chemistry · 2020Article
- The Model SystemLife (Basel, Switzerland) · 2020Review
- Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme fromBiotechnology for biofuels · 2020Article
- Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris.BMC research notes · 2019Article
- Structure-function analyses reveal that a glucuronoyl esterase fromThe Journal of biological chemistry · 2019Article
- Biochemical and structural features of diverse bacterial glucuronoyl esterases facilitating recalcitrant biomass conversion.Biotechnology for biofuels · 2018Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The glucuronoyl esterases (GEs) that have been identified so far belong to family 15 of the carbohydrate esterases in the CAZy classification system and are presumed to target ester bonds between lignin alcohols and (4-O-methyl-)D-glucuronic acid residues of xylan. Few GEs have been cloned, expressed and characterised to date. Characterisation has been done on a variety of synthetic substrates; however, the number of commercially available substrates is very limited. We identified novel putative GEs from a wide taxonomic range of fungi and expressed the enzymes originating from Acremonium alcalophilum and Wolfiporia cocos as well as the previously described PcGE1 from Phanerochaete chrysosporium. All three fungal GEs were active on the commercially available compounds benzyl glucuronic acid (BnGlcA), allyl glucuronic acid (allylGlcA) and to a lower degree on methyl glucuronic acid (MeGlcA). The enzymes showed pH stability over a wide pH range and tolerated 6-h incubations of up to 50 °C. Kinetic parameters were determined for BnGlcA. This study shows the suitability of the commercially available model compounds BnGlcA, MeGlcA and allylGlcA in GE activity screening and characterisation experiments. We enriched the spectrum of characterised GEs with two new members of a relatively young enzyme family. Due to its biotechnological significance, this family deserves to be more extensively studied. The presented enzymes are promising candidates as auxiliary enzymes to improve saccharification of plant biomass.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.