ArticleClinical and experimental reproductive medicine2017
A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser.
Article in Clinical and experimental reproductive medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Comparison of intracytoplasmic sperm injection outcome with fresh versus frozen-thawed testicular sperm in men with nonobstructive azoospermia: a systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2018Pooled it
- Sperm selection for assisted reproductive technology: do we have a winner?Journal of assisted reproduction and genetics · 2025Review
- Sperm cryopreservation protocol for micro-TESE-retrieved sperm.Asian journal of andrology · 2025Review
- A narrative review of sperm selection technology for assisted reproduction techniques.Translational andrology and urology · 2024Review
- Male Infertility: New Developments, Current Challenges, and Future Directions.The world journal of men's health · 2024Review
- Laser-Guided Sperm Selection: Optimizing the Reproductive Success Rate in Assisted Reproductive Technology.Cureus · 2023Review
- Applications of laser technology in the manipulation of human spermatozoa.Reproductive biology and endocrinology : RB&E · 2023Review
- Laser-assisted selection of immotile spermatozoa has no effect on obstetric and neonatal outcomes of TESA-ICSI pregnancies.Reproductive biology and endocrinology : RB&E · 2021Article
- Diagnostics and Management of Male Infertility in Primary Ciliary Dyskinesia.Diagnostics (Basel, Switzerland) · 2021Review
- Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA.Journal of clinical medicine · 2021Review
- Postmortem Sperm Retrieval in Hanging-Induced Mechanical Asphyxia: Probability and Temporal Dynamics.Journal of human reproductive sciencesArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39.8% was detected using the laser. In all, five mature oocytes were injected, resulting in four cases of normal fertilization (80%) on day 1. Further, two high-quality day 3 embryos were transferred, which resulted in a singleton pregnancy. Our study demonstrates that completely immotile spermatozoa are worth cryopreserving for further intracytoplasmic sperm injection, which provides a new insight into male fertility preservation in cases of completely immotile spermatozoa.
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