ArticlePlant methods2017
Reference genes for normalization of qPCR assays in sugarcane plants under water deficit.
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
- Selection and validation of stable reference genes in potato infected by Pectobacterium atrosepticum using real-time quantitative PCR.Scientific reports · 2025Article
- Identification and expression analysis of nuclear factor Y transcription factor genes under drought, cold and Eldana infestation in sugarcane (Saccharum spp. hybrid).Genes & genomics · 2024Article
- Comparative Analysis of Sucrose-Regulatory Genes in High- and Low-Sucrose Sister Clones of Sugarcane.Plants (Basel, Switzerland) · 2024Article
- Evaluation of barley genotypes for drought adaptability: based on stress indices and comprehensive evaluation as criteria.Frontiers in plant science · 2024Article
- Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets.Plants (Basel, Switzerland) · 2023Article
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- Analysis of the PEBP gene family and identification of a novel FLOWERING LOCUS T orthologue in sugarcane.Journal of experimental botany · 2022Article
- Genetic manipulation of trehalose-6-phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems.Plant direct · 2021Article
- Role of bundle sheath conductance in sustaining photosynthesis competence in sugarcane plants under nitrogen deficiency.Photosynthesis research · 2021Article
- hRNAi-mediated knock-down of Sphenophorus levis V-ATPase E in transgenic sugarcane (Saccharum spp interspecific hybrid) affects the insect growth and survival.Plant cell reports · 2021Article
- Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.).Scientific reports · 2021Article
- Selection of reference genes for normalization of microRNA expression in sugarcane stalks during its interaction with3 Biotech · 2021Article
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- Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum.Scientific reports · 2020Article
- Selection and validation of reliable reference genes for gene expression studies from Monilinia vaccinii-corymbosi infected wild blueberry phenotypes.Scientific reports · 2020Article
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- Expression Analysis of XTH in Stem Swelling of Stem Mustard and Selection of Reference Genes.Genes · 2020Article
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Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSugarcane (
resultsIn this study, candidate genes were tested for their suitable as reference genes for qPCR analyses in two sugarcane cultivars with varying drought tolerance. Eight candidate reference genes were evaluated in leaves sampled in plants subjected to water deficit in both field and greenhouse conditions. In addition, five genes were evaluated in shoot roots of plants subjected to water deficit by adding PEG8000 to the nutrient solution. NormFinder and RefFinder algorithms were used to identify the most stable gene(s) among genotypes and under different experimental conditions. Both algorithms revealed that in leaf samples,
conclusionReference genes suitable for sugarcane under water deficit were identified, which would lead to a more accurate and reliable analysis of qPCR. Thus, results obtained in this study may guide future research on gene expression in sugarcane under varying water conditions.
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