ArticleFrontiers in physiology2017
Morphological Regeneration and Functional Recovery of Neuromuscular Junctions after Tourniquet-Induced Injuries in Mouse Hindlimb.
Article in Frontiers in physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- [Prehospital application of tourniquets for life-threatening extremity hemorrhage : Systematic review of literature].Der Unfallchirurg · 2018Pooled it
- Fibro-Adipogenic Progenitors Regulate Orofacial Neuromuscular Junction Regeneration via Myostatin.Journal of cachexia, sarcopenia and muscle · 2026Article
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- Phase-Specific Evaluation of Sciatic Nerve Regeneration in Preclinical Studies: A Review of Functional Assessment, Emerging Therapies, and Translational Value.International journal of molecular sciences · 2025Review
- Muscle atrophy and fibrosis are attenuated after experimental nerve repair associated with heterologous fibrin biopolymer.Regenerative medicine · 2025Article
- Neuromuscular Junction Damage in the Calf Muscles of Patients With Advanced Peripheral Artery Disease.Neuropathology and applied neurobiology · 2025Article
- Review
- Postnatal Protein Intake as a Determinant of Skeletal Muscle Structure and Function in Mice-A Pilot Study.International journal of molecular sciences · 2022Article
- Different responses of skeletal muscles to femoral artery ligation-induced ischemia identified in BABL/c and C57BL/6 mice.Frontiers in physiology · 2022Article
- Secondary denervation is a chronic pathophysiologic sequela of volumetric muscle loss.Journal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor.Frontiers in physiology · 2021Article
- Review
- Remnant neuromuscular junctions in denervated muscles contribute to functional recovery in delayed peripheral nerve repair.Neural regeneration research · 2020Article
- Evaluation of a conducting elastomeric composite material for intramuscular electrode application.Acta biomaterialia · 2020Article
- Plasma flow distal to tourniquet placement provides a physiological mechanism for tissue salvage.PloS one · 2020Article
- Effects of ASC Application on Endplate Regeneration Upon Glycerol-Induced Muscle Damage.Frontiers in molecular neuroscience · 2020Article
- Review
- Voluntary running protects against neuromuscular dysfunction following hindlimb ischemia-reperfusion in mice.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- AMP-Activated Protein Kinase as a Key Trigger for the Disuse-Induced Skeletal Muscle Remodeling.International journal of molecular sciences · 2018Review
- NMJ maintenance and repair in aging.Current opinion in physiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tourniquet application and its subsequent release cause serious injuries to the skeletal muscle, nerve, and neuromuscular junction (NMJ) due to mechanical compression and ischemia-reperfusion (IR). Monitoring structural and functional repair of the NMJ, nerve, and skeletal muscle after tourniquet-induced injuries is beneficial in exploring potential cellular and molecular mechanisms responsible for tourniquet-induced injuries, and for establishing effective therapeutic interventions. Here, we observed long-term morphological and functional changes of the NMJ in a murine model of tourniquet-induced hindlimb injuries. Unilateral hindlimbs of C57/BL6 mice were subjected to 3 h of tourniquet by placing an orthodontic rubber band, followed by varied periods of tourniquet release (1 day, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks). NMJ morphology in the gastrocnemius muscle was imaged, and the endplate potential (EPP) was recorded to evaluate NMJ function. In NMJs, nicotinic acetylcholine receptor (nAChR) clusters normally displayed an intact, pretzel-like shape, and all nAChR clusters were innervated (100%) by motor nerve terminals. During 3 h of tourniquet application and varied periods of tourniquet release, NMJs in the gastrocnemius muscle were characterized by morphological and functional changes. At 1 day and 3 days of tourniquet release, nAChR clusters retained normal, pretzel-like shapes, whereas motor nerve terminals were completely destroyed and no EPPs recorded. From 1 to 6 weeks of tourniquet release, motor nerve terminals gradually regenerated, even reaching that seen in sham mice, whereas nAChR clusters were gradually fragmented with prolongation of tourniquet release. Additionally, the amplitude of EPPs gradually increased with prolongation of tourniquet release. However, even at 6 weeks after tourniquet release, the amplitude of EPPs did not restore to the level seen in sham mice (13.9 ± 1.1 mV,
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