ArticleNeuron2017
iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.
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Article in Neuron, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 731 papers.
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731citing papers in PubMed
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731 citing papers in PubMed, 1,136 citations in OpenAlex.
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- Human Stem Cell-Derived Models of the Alzheimer's Disease Neuroimmune System.International journal of molecular sciences · 2026Review
- Identification of a NUAK1/2 Inhibitor as a Macrophage-Polarizing Compound by a Machine Learning-Based Phenotypic Cell Painting Screen.International journal of molecular sciences · 2026Article
671 more citing papers are in PubMed but not listed here.
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Authors and funding
23 authors at 6 institutions in 2 countries.
Edsel M AbudDepartment of Neurobiology & Behavior, University of California Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA; Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA.
Ricardo N RamirezDepartment of Developmental and Cell Biology, University of California Irvine, Irvine, CA 92697, USA.
Eric S MartinezDepartment of Neurobiology & Behavior, University of California Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA; Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA.
Luke M HealyNeuroimmunology Unit, Department of Neurology and Neurosurgery, Montreal Neurological Institute and Hospital, McGill University, Montreal, QC H3A 2B4, Canada.
Cecilia H H NguyenDepartment of Neurobiology & Behavior, University of California Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA; Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA.
Sean A NewmanSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA.
Andriy V YerominDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA 92697, USA.
Vanessa M ScarfoneSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA.
Samuel E MarshSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA; Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA.
Cristhian FimbresInstitute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA.
Chad A CarawayInstitute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA.
Gianna M FoteDepartment of Neurobiology & Behavior, University of California Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA; Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA.
Abdullah M MadanyDivision of Biomedical Sciences, Center for Glia-Neuronal Interactions, University of California, Riverside, Riverside, CA 92521, USA.
Anshu AgrawalDepartment of Medicine, School of Medicine, University of California Irvine, Irvine, CA 92697, USA.
Rakez KayedDepartment of Neurology, George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
Karen H GylysUCLA School of Nursing, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Michael D CahalanDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA 92697, USA.
Brian J CummingsSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA; Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA; Anatomy and Neurobiology, University of California Irvine, Irvine, CA 92697, USA.
Jack P AntelNeuroimmunology Unit, Department of Neurology and Neurosurgery, Montreal Neurological Institute and Hospital, McGill University, Montreal, QC H3A 2B4, Canada.
Ali MortazaviDepartment of Developmental and Cell Biology, University of California Irvine, Irvine, CA 92697, USA.
Monica J CarsonDivision of Biomedical Sciences, Center for Glia-Neuronal Interactions, University of California, Riverside, Riverside, CA 92521, USA.
Wayne W PoonInstitute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA. Electronic address: wpoon@uci.edu.
Mathew Blurton-JonesDepartment of Neurobiology & Behavior, University of California Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA; Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697, USA. Electronic address: mblurton@uci.edu.
University of California, Irvine · USUniversity of California, Riverside · USMcGill University · CAMontreal Neurological Institute and Hospital · CAThe University of Texas Medical Branch at Galveston · USUniversity of California, Los Angeles · US
Funding
Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Christine Hui · 1994 to 2026
$57.9MVASCULAR RISK AND COGNITIVE STATUS IN A LATINO POPULATIONP50AG005142 · NIA · UNIVERSITY OF SOUTHERN CALIFORNIA · PI ARANDA, MARIA P. · 1985 to 2019
$41.0MUC Irvine Alzheimer's Disease Research Center Induced Pluripotent Stem Cell CoreP50AG016573 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI LAFERLA, FRANK M · 2000 to 2019
$37.0MUniversity of California Health Participation in the National COVID Cohort Collaborative (N3C)UL1TR001414 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI COOPER, DAN M, VILAIN, ERIC J. · 2015 to 2023
$35.1MTISSUE REPOSITORY COREP01AG000538 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI LAFERLA, FRANK M · 1985 to 2018
$24.8MMEDICAL SCIENTIST TRAINING PROGRAMT32GM008620 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GOLDIN, ALAN L · 1999 to 2023
$8.1MFormation and Propagation of Tau Oligomeric Strains in Alzheimer's DiseaseR01AG054025 · NIA · UNIVERSITY OF TEXAS MED BR GALVESTON · PI Rakez Kayed · 2016 to 2026
$5.8MCellular and Molecular Mechanisms of Regulatory T Cells in EAER01AI121945 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI CAHALAN, MICHAEL D · 2016 to 2020
$2.5MThe role of TREM2 in human microglial function and Alzheimer disease pathogenesisRF1AG048099 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI BLURTON-JONES, MATHEW MARK · 2014 to 2014
$1.9MApoE and cholesterol effects on amyloid beta in synaptic terminals in ADR56AG027465 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GYLYS, KAREN H · 2015 to 2015
$385kNCATS NIH HHS UL1 TR001414NCI NIH HHS P30 CA062203NIAID NIH HHS R01 AI121945NIA NIH HHS P01 AG000538NIA NIH HHS P50 AG005142NIA NIH HHS P50 AG016573NIA NIH HHS R01 AG054025NIA NIH HHS R56 AG027465NIA NIH HHS RF1 AG048099NIGMS NIH HHS T32 GM008620
Abstract
Microglia play critical roles in brain development, homeostasis, and neurological disorders. Here, we report that human microglial-like cells (iMGLs) can be differentiated from iPSCs to study their function in neurological diseases, like Alzheimer's disease (AD). We find that iMGLs develop in vitro similarly to microglia in vivo, and whole-transcriptome analysis demonstrates that they are highly similar to cultured adult and fetal human microglia. Functional assessment of iMGLs reveals that they secrete cytokines in response to inflammatory stimuli, migrate and undergo calcium transients, and robustly phagocytose CNS substrates. iMGLs were used to examine the effects of Aβ fibrils and brain-derived tau oligomers on AD-related gene expression and to interrogate mechanisms involved in synaptic pruning. Furthermore, iMGLs transplanted into transgenic mice and human brain organoids resemble microglia in vivo. Together, these findings demonstrate that iMGLs can be used to study microglial function, providing important new insight into human neurological disease.
Indexed as
Alzheimer DiseaseAmyloid beta-PeptidesAmyloid beta-Protein PrecursorAnimalsBrainCells, CulturedCytokinesDisease Models, AnimalHumansInduced Pluripotent Stem CellsMiceMicrogliaPeptide FragmentsAmyloid beta-PeptidesAmyloid beta-Protein PrecursorCytokinesPeptide Fragments3D organoidsAD-GWASAlzheimer’s diseaseBeta-amyloidcell models of diseaseinduced pluripotent stem cellsmicrogliamouse transplantationneurodegenerative diseasesTau
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