ArticleNature protocols2017
Modular low-light microscope for imaging cellular bioluminescence and radioluminescence.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Radioluminescence microscopy of tumor organoids enabled by a reconfigurable high-resolution imaging setup.EJNMMI research · 2026Article
- A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging.Nature methods · 2025Article
- Method for optimizing imaging parameters to record neuronal and cellular activity at depth with bioluminescence.Neurophotonics · 2024Article
- Increased [Npj imaging · 2024Article
- Development of a Lensless Radiomicroscope for Cellular-Resolution Radionuclide Imaging.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2023Article
- Heterologous reporter expression in the planarianCell reports methods · 2022Article
- Chemiluminescent spiroadamantane-1,2-dioxetanes: Recent advances in molecular imaging and biomarker detection.Current opinion in chemical biology · 2022Review
- Advancing Tumor Microenvironment Research by Combining Organs-on-Chips and Biosensors.Advances in experimental medicine and biology · 2022Review
- Flow radiocytometry using droplet optofluidics.Biosensors & bioelectronics · 2021Article
- Electric Field Tunability of Photoluminescence from a Hybrid Peptide-Plasmonic Metal Microfabricated Chip.JACS Au · 2021Article
- High-resolution positron emission microscopy of patient-derived tumor organoids.Nature communications · 2021Article
- High-resolution radioluminescence microscopy of FDG uptake in an engineered 3D tumor-stoma model.European journal of nuclear medicine and molecular imaging · 2021Article
- Cardioluminescence in Transgenic Zebrafish Larvae: A Calcium Imaging Tool to Study Drug Effects and Pathological Modeling.Biomedicines · 2021Article
- Microfluidics-Coupled Radioluminescence Microscopy forAnalytical chemistry · 2021Article
- Single-cell radioluminescence microscopy with two-fold higher sensitivity using dual scintillator configuration.PloS one · 2020Article
- Visualization of Mitochondrial CaInternational journal of molecular sciences · 2019Article
- Thermochemiluminescent peroxide crystals.Nature communications · 2019Article
- Radioluminescence in biomedicine: physics, applications, and models.Physics in medicine and biology · 2019Review
- Lactic Acid Accumulation in the Tumor Microenvironment SuppressesCancer research · 2019Article
- Multiplexed Single-Cell Measurements of FDG Uptake and Lactate Release Using Droplet Microfluidics.Technology in cancer research & treatment · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Low-light microscopy methods are receiving increased attention as new applications have emerged. One such application is to allow longitudinal imaging of light-sensitive cells with no phototoxicity and no photobleaching of fluorescent biomarkers. Another application is for imaging signals that are inherently dim and undetectable using standard microscopy techniques, such as bioluminescence, chemiluminescence or radioluminescence. In this protocol, we provide instructions on how to build a modular low-light microscope (1-4 d) by coupling two microscope objective lenses, back to back from each other, using standard optomechanical components. We also provide directions on how to image dim signals such as those of radioluminescence (1-1.5 h), bioluminescence (∼30 min) and low-excitation fluorescence (∼15 min). In particular, radioluminescence microscopy is explained in detail, as it is a newly developed technique that enables the study of small-molecule transport (e.g., radiolabeled drugs, metabolic precursors and nuclear medicine contrast agents) by single cells without perturbing endogenous biochemical processes. In this imaging technique, a scintillator crystal (e.g., CdWO
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.