ArticleToxins2017
A Simple and Specific Noncompetitive ELISA Method for HT-2 Toxin Detection.
Article in Toxins, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Antibody-based sensing of pesticide residues and mycotoxins in plant-derived foods: A narrative review of ELISA, lateral-flow immunoassays and immunosensors.Food chemistry: X · 2026Review
- Biotechnology-enhanced immunoassay for accurate determination of HT-2 toxin in edible insect samples.Mikrochimica acta · 2025Article
- Review
- Detection of estradiol with a digital immunoassay using an anti-immunocomplex antibody and single-molecule observation of gold nanoparticles.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- The Potential of Single-Chain Variable Fragment Antibody: Role in Future Therapeutic and Diagnostic Biologics.Journal of immunology research · 2024Review
- A universal CRISPR-Cas14a responsive triple-sensitized upconversion photoelectrochemical sensor.Journal of nanobiotechnology · 2023Article
- Phage display technology for fabricating a recombinant monoclonal ScFv antibody against tetanus toxin.Toxicology research · 2023Article
- Review
- Rapid Detection ofMolecules (Basel, Switzerland) · 2022Article
- Developing of specific monoclonal recombinant antibody fused to alkaline phosphatase (AP) for one-step detection of fig mosaic virus.3 Biotech · 2022Article
- Recombinant antibodies and their use for food immunoanalysis.Analytical and bioanalytical chemistry · 2022Review
- Development of a sensitive non-competitive immunoassay via immunocomplex binding peptide for the determination of ethyl carbamate in wine samples.Journal of hazardous materials · 2021Article
- Layered material platform for surface plasmon resonance biosensing.Scientific reports · 2019Article
- Enhancing the detection ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Competitive fluorometric assay for the food toxin T-2 by using DNA-modified silver nanoclusters, aptamer-modified magnetic beads, and exponential isothermal amplification.Mikrochimica acta · 2019Article
- Nanobody Technology for Mycotoxin Detection in the Field of Food Safety: Current Status and Prospects.Toxins · 2018Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed an HT-2 toxin-specific simple ELISA format with a positive read-out. The assay is based on an anti-immune complex (IC) scFv antibody fragment, which is genetically fused with alkaline phosphatase (AP). The anti-IC antibody specifically recognizes the IC between a primary anti-HT-2 toxin Fab fragment and an HT-2 toxin molecule. In the IC ELISA format, the sample is added together with the scFv-AP antibody to the ELISA plate coated with the primary antibody. After 15 min of incubation and a washing step, the ELISA response is read. A competitive ELISA including only the primary antibody recognizes both HT-2 and T-2 toxins. The anti-IC antibody makes the assay specific for HT-2 toxin, and the IC ELISA is over 10 times more sensitive compared to the competitive assay. Three different naturally contaminated matrices: wheat, barley and oats, were used to evaluate the assay performance with real samples. The corresponding limits of detection were 0.3 ng/mL (13 µg/kg), 0.1 ng/mL (4 µg/kg) and 0.3 ng/mL (16 µg/kg), respectively. The IC ELISA can be used for screening HT-2 toxin specifically and in relevant concentration ranges from all three tested grain matrices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.