ArticleScientific reports2017
Elucidation of quantitative structural diversity of remarkable rearrangement regions, shufflons, in IncI2 plasmids.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Genomic evolution and stability of the mcr-1-harboring IncI2 plasmid in the presence and absence of colistin.BMC genomics · 2025Article
- Do the Shuffle: Expanding the Synthetic Biology Toolkit for Shufflon-like Recombination Systems.ACS synthetic biology · 2025Article
- RSYD-BASIC: a bioinformatic pipeline for routine sequence analysis and data processing of bacterial isolates for clinical microbiology.Access microbiology · 2025Article
- New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates.PloS one · 2024Article
- Dihydroartemisinin inhibits plasmid transfer in drug-resistantZoological research · 2023Article
- Systematic identification of gene-altering programmed inversions across the bacterial domain.Nucleic acids research · 2023Article
- The Type IV Pilus of Plasmid TP114 Displays Adhesins Conferring Conjugation Specificity and Is Important for DNA Transfer in the Mouse Gut Microbiota.Microbiology spectrum · 2022Article
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- Successful Dissemination of Plasmid-Mediated Extended-Spectrum β-Lactamases in Enterobacterales over Humans to Wild Fauna.Microorganisms · 2021Article
- Association between theAntibiotics (Basel, Switzerland) · 2021Article
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- Standardized Natural Citrus Extract dietary supplementation influences sows' microbiota, welfare, and preweaning piglets' performances in commercial rearing conditions.Translational animal science · 2020Article
- Selective and non-selective bottlenecks as drivers of the evolution of hypermutable bacterial loci.Molecular microbiology · 2020Review
- Metadata Analysis ofFrontiers in microbiology · 2020Article
- Transferable Plasmids ofFrontiers in microbiology · 2020Review
- Enterohaemorrhagic Escherichia coli O121:H19 acquired an extended-spectrum β-lactamase gene during the development of an outbreak in two nurseries.Microbial genomics · 2019Article
- Characterization of a novelInfection and drug resistance · 2019Article
- Mobile Genetic Elements Associated with Antimicrobial Resistance.Clinical microbiology reviews · 2018Review
- Pushing the limits of de novo genome assembly for complex prokaryotic genomes harboring very long, near identical repeats.Nucleic acids research · 2018Article
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Authors and funding
9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A multiple DNA inversion system, the shufflon, exists in incompatibility (Inc) I1 and I2 plasmids. The shufflon generates variants of the PilV protein, a minor component of the thin pilus. The shufflon is one of the most difficult regions for de novo genome assembly because of its structural diversity even in an isolated bacterial clone. We determined complete genome sequences, including those of IncI2 plasmids carrying mcr-1, of three Escherichia coli strains using single-molecule, real-time (SMRT) sequencing and Illumina sequencing. The sequences assembled using only SMRT sequencing contained misassembled regions in the shufflon. A hybrid analysis using SMRT and Illumina sequencing resolved the misassembled region and revealed that the three IncI2 plasmids, excluding the shufflon region, were highly conserved. Moreover, the abundance ratio of whole-shufflon structures could be determined by quantitative structural variation analysis of the SMRT data, suggesting that a remarkable heterogeneity of whole-shufflon structural variations exists in IncI2 plasmids. These findings indicate that remarkable rearrangement regions should be validated using both long-read and short-read sequencing data and that the structural variation of PilV in the shufflon might be closely related to phenotypic heterogeneity of plasmid-mediated transconjugation involved in horizontal gene transfer even in bacterial clonal populations.
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