ArticleScientific reports2017
TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- XPO1 R749Q Mutations Co-occur with POLE Mutations in Cancer and Can Be Targeted to Overcome Chemoresistance.Cancer research · 2025Article
- Closing the multichannel gap through computational reconstruction of interaction in super-resolution microscopy.Patterns (New York, N.Y.) · 2025Review
- Cluster parameter-based DBSCAN maps for image characterization.Computational and structural biotechnology journal · 2025Article
- Triggered cagedSTORM microscopy.Biomedical optics express · 2024Article
- Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Development of AI-assisted microscopy frameworks through realistic simulation with pySTED.Nature machine intelligence · 2024Article
- Simulating structurally variable nuclear pore complexes for microscopy.Bioinformatics (Oxford, England) · 2023Article
- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging.Nature methods · 2023Article
- Single molecule imaging simulations with advanced fluorophore photophysics.Communications biology · 2023Article
- Application of Lacunarity for Quantification of Single Molecule Localization Microscopy Images.Cells · 2022Article
- upU-Net Approaches for Background Emission Removal in Fluorescence Microscopy.Journal of imaging · 2022Article
- Technological advances in super-resolution microscopy to study cellular processes.Molecular cell · 2022Review
- Corrected Super-Resolution Microscopy Enables Nanoscale Imaging of Autofluorescent Lung Macrophages.Biophysical journal · 2020Article
- A Review of Super-Resolution Single-Molecule Localization Microscopy Cluster Analysis and Quantification Methods.Patterns (New York, N.Y.) · 2020Review
- Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.Journal of fluorescence · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optimization of sample, imaging and data processing parameters is an essential task in localization based super-resolution microscopy, where the final image quality strongly depends on the imaging of single isolated fluorescent molecules. A computational solution that uses a simulator software for the generation of test data stacks was proposed, developed and tested. The implemented advanced physical models such as scalar and vector based point spread functions, polarization sensitive detection, drift, spectral crosstalk, structured background etc., made the simulation results more realistic and helped us interpret the final super-resolved images and distinguish between real structures and imaging artefacts.
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Registered trials
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