ArticleScientific reports2017
Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Coelenterazine Analogs for Bioassays and Molecular Imaging.Sensors (Basel, Switzerland) · 2025Review
- Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins.Sensors (Basel, Switzerland) · 2023Article
- Bright Molecular Strain Probe Templates for Reporting Protein-Protein Interactions.Sensors (Basel, Switzerland) · 2023Article
- S-Series Coelenterazine-Driven Combinatorial Bioluminescence Imaging Systems for Mammalian Cells.International journal of molecular sciences · 2023Article
- Near-Infrared Imaging of Steroid Hormone Activities Using Bright BRET Templates.International journal of molecular sciences · 2022Article
- C-Series Coelenterazine-Driven Bioluminescence Signature Imaging.International journal of molecular sciences · 2022Article
- Multiplex quadruple bioluminescent assay system.Scientific reports · 2022Article
- Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Bioluminescent Imaging Systems for Assay Developments.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Review
- Color-tunable bioluminescence imaging portfolio for cell imaging.Scientific reports · 2021Article
- Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- Highly bright and stable NIR-BRET with blue-shifted coelenterazine derivatives for deep-tissue imaging of molecular eventsTheranostics · 2019Article
- Advances in bioluminescence imaging: new probes from old recipes.Current opinion in chemical biology · 2018Review
- Parallel Screening for Rapid Identification of Orthogonal Bioluminescent Tools.ACS central science · 2017Article
- Bioluminescent Probes for Imaging Biology beyond the Culture Dish.Biochemistry · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luciferase selectivity. In the attempt, we found that CTZ analogues with ethynyl or styryl groups display dramatically biased bioluminescence to specific luciferases and pHs by modifying the functional groups at the C-2 and C-6 positions of the imidazopyradinone backbone of CTZ. The optical contamination-free feature was exemplified with the luciferase-specific CTZ analogues, which illuminated anti-estrogenic and rapamycin activities in a mixture of optical probes. This unique bioluminescence platform has great potential for specific and high throughput imaging of multiple optical readouts in bioassays without optical contamination.
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