ArticleScientific reports2017
Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 112 citations in OpenAlex.
- A novel needleless delivery of Cas9 ribonucleoprotein complex in Bemisia tabaci embryos for gene editing.World journal of microbiology & biotechnology · 2026Article
- Selective Breeding and Trait Improvement of Insect Natural Enemies for Ecologically Robust Biological Control.Insects · 2026Review
- Review
- The jewel wasp Nasonia vitripennis utilizes two single-copy, protamine-like sperm nuclear basic proteins.G3 (Bethesda, Md.) · 2026Article
- Genomic landscape and genetic manipulation of an ectoparasitoid wasp, Gregopimpla kuwanae.Communications biology · 2026Article
- SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis.Insect molecular biology · 2026Article
- Why just fly?Fly · 2025Review
- Pioneering genome editing in parthenogenetic stick insects: CRISPR/Cas9-mediated gene knockout in Medauroidea extradentata.Scientific reports · 2025Article
- Manipulation of a New Non-model Insect Genome Using Targeted CRISPR-Era Approaches.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Uncovering the circadian transcriptome ofProceedings. Biological sciences · 2024Article
- Direct Parental (DIPA) CRISPR in the jewel wasp, Nasonia vitripennis.G3 (Bethesda, Md.) · 2024Article
- Identification of Parthenogenesis-Inducing Effector Proteins in Wolbachia.Genome biology and evolution · 2024Article
- The Development and Expansion of in vivo Germline Editing Technologies in Arthropods: Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) and Beyond.Integrative and comparative biology · 2023Review
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- Genetic modification of the ant Lasius niger using CRISPR-Cas9 technology.Insect molecular biology · 2023Article
- Article
- Venom biotechnology: casting light on nature's deadliest weapons using synthetic biology.Frontiers in bioengineering and biotechnology · 2023Review
- TheEcology and evolution · 2023Review
- Melanin Synthesis Pathway Interruption: CRISPR/Cas9-mediated Knockout of dopa decarboxylase (DDC) in Harmonia axyridis (Coleoptera: Coccinellidae).Journal of insect science (Online) · 2022Article
- Modern venomics-Current insights, novel methods, and future perspectives in biological and applied animal venom research.GigaScience · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The revolutionary RNA-guided endonuclease CRISPR/Cas9 system has proven to be a powerful tool for gene editing in a plethora of organisms. Here, utilizing this system we developed an efficient protocol for the generation of heritable germline mutations in the parasitoid jewel wasp, Nasonia vitripennis, a rising insect model organism for the study of evolution, development of axis pattern formation, venom production, haplo-diploid sex determination, and host-symbiont interactions. To establish CRISPR-directed gene editing in N. vitripennis, we targeted a conserved eye pigmentation gene cinnabar, generating several independent heritable germline mutations in this gene. Briefly, to generate these mutants, we developed a protocol to efficiently collect N. vitripennis eggs from a parasitized flesh fly pupa, Sarcophaga bullata, inject these eggs with Cas9/guide RNA mixtures, and transfer injected eggs back into the host to continue development. We also describe a flow for screening mutants and establishing stable mutant strains through genetic crosses. Overall, our results demonstrate that the CRISPR/Cas9 system is a powerful tool for genome manipulation in N. vitripennis, with strong potential for expansion to target critical genes, thus allowing for the investigation of several important biological phenomena in this organism.
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