Evidence map›Paper›PMID 28423355›Full record

ArticleOncotarget2017

Mesenchymal stem cells release exosomes that transfer miRNAs to endothelial cells and promote angiogenesis.

Min Gong, Bin Yu, Jingcai Wang, Yigang Wang, Min Liu, Christian Paul, Ronald W Millard, De-Sheng Xiao, Muhammad Ashraf, Meifeng Xu

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 257 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
257citing papers in PubMed, 2 pooled it
15.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

257 citing papers in PubMed, 2 syntheses or guidelines pooled it, 389 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Trial
  4. Trial
  5. Extracellular Vesicles and Their Role in Osteogenesis.Bioengineering (Basel, Switzerland) · 2026
    Review
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197 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Min GongDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Bin YuDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Jingcai WangDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Yigang WangDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Min LiuDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Christian PaulDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Ronald W MillardDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
De-Sheng XiaoDepartment of Preventive Medicine, School of Public Health, Guangzhou Medical University, Guangzhou, Guangdong Province, China.
Muhammad AshrafDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Meifeng XuDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
University of Cincinnati · USUniversity of Cincinnati Medical Center · USChildren's Hospital of Chongqing Medical University · CN

Funding

Integration Free IPS Cells-Derived Progenitors for Cardiac RegenerationR01HL126516 · NHLBI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI REHMAN, JALEES · 2015 to 2019
$3.0M
MicroRNA as mediators of angiogenesis & ischemic myocardial repairR01HL114654 · NHLBI · UNIVERSITY OF CINCINNATI · PI ASHRAF, MUHAMMAD, XU, MEIFENG · 2012 to 2016
$2.5M
The therapeutic potential of smarter exosomes derived from engineered MSCs on Alzheimer's diseaseR01HL140962 · NHLBI · UNIVERSITY OF CINCINNATI · PI LIU, MIN, XU, MEIFENG · 2018 to 2021
$2.4M
Wnt11 signaling in stem cell survival and cardiac regenerationR01HL105176 · NHLBI · UNIVERSITY OF CINCINNATI · PI ASHRAF, MUHAMMAD, JONES, WALTER KEITH · 2010 to 2014
$1.9M
NHLBI NIH HHS R01 HL105176NHLBI NIH HHS R01 HL114654NHLBI NIH HHS R01 HL126516NHLBI NIH HHS R01 HL140962
6 · The paper itself

Abstract

Mesenchymal stem cells (MSCs) have been found to benefit patients with a variety of ischemic diseases via promoting angiogenesis. It is also well established that exosomes secreted from MSCs deliver bioactive molecules, including microRNAs (miRs) to recipient cells. Therefore, we hypothesized that exosomes secreted from MSCs deliver miRs into endothelial cells and mediate angiogenesis. The pro-angiogenic stimulatory capacity of exosomes was investigated using tube-like structure formation and spheroid-based sprouting of human umbilical vein endothelial cells (HUVECs), and in vivo Matrigel plug assay. The secretion of pro-angiogenic miRs (pro-angiomiRs) from MSCs into culture medium and transfer of the miRs to HUVECs were confirmed using real-time quantitative PCR. Supplementation of the exosome secretion blocker GW4869 (10 μM) reduced the pro-angiomiRs in the MSC-derived conditioned medium (CdMMSC). Addition of exosomes isolated from CdMMSC could directly 1) promote HUVEC tube-like structure formation in vitro; 2) mobilize endothelial cells into Matrigel plug subcutaneously transplanted into mice; and 3) increase blood flow inside Matrigel plug. Fluorescence tracking showed that the exosomes were internalized rapidly by HUVECs causing an upregulated expression of pro-angiomiRs in HUVECs. Loss-and-gain function of the pro-angiomiRs (e.g., miR-30b) in MSCs significantly altered the pro-angiogenic properties of these MSC-derived exosomes, which could be associated with the regulation of their targets in HUVECs. These results suggest that exosomal transfer of pro-angiogenic miRs plays an important role in MSC mediated angiogenesis and stem cell-to-endothelial cell communication.

Indexed as

AnimalsEndothelial CellsExosomesHumansHuman Umbilical Vein Endothelial CellsMesenchymal Stem CellsMiceMice, Inbred C57BLMicroRNAsNeovascularization, PhysiologicSignal TransductionMicroRNAsangiogenesisexosomesmesenchymal stem cellsmiR-30bmiRNA transfer

Identifiers

PMID28423355
PMCPMC5542178
OpenAlexW2601082158

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.