ArticleCell reports2017
Rapid Molecular Profiling of Defined Cell Types Using Viral TRAP.
Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.
- Functional genomic mechanisms of opioid action and opioid use disorder: a systematic review of animal models and human studies.Molecular psychiatry · 2023Pooled it
- Activity-dependent ribosome profiling reveals the landscape of canonical and non-canonical translation in brain tissue.Nature communications · 2026Article
- A pathway from the anterior cingulate cortex to the lateral habenula controls chronic pain-induced depression in male mice.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Article
- A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types.Cell reports · 2026Article
- 4E-BP2-dependent translational control in GABAergic interneurons is required for long-term memory.Molecular neurobiology · 2026Article
- A prelimbic molecular clock of protein synthesis for memory persistence.bioRxiv : the preprint server for biology · 2026Article
- Toward a Genetic Signature of Resistance to Activity-Based Anorexia in Striatal Projecting Cortical Neurons.The International journal of eating disorders · 2025Article
- Thalamo-hippocampal pathway determines aggression and self-harm.Science advances · 2025Article
- A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types.Research square · 2025Article
- Article
- Changes in nucleus accumbens core translatome accompanying incubation of cocaine craving.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2025Article
- Molecular signatures of regional vulnerability to tauopathy in excitatory cortical neurons.Acta neuropathologica · 2025Article
- Direct interoceptive input to the insular cortex shapes learned feeding behavior.bioRxiv : the preprint server for biology · 2025Article
- Deep mining in the dorsal raphe: cholecystokinin-expressing neurons encode satiation-related cues to regulate meal size.Signal transduction and targeted therapy · 2025Article
- Article
- Neuronal and Molecular Mechanisms Underlying Chronic Pain and Depression Comorbidity in the Paraventricular Thalamus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- RGS4 Actions in Mouse Prefrontal Cortex Modulate Behavioral and Transcriptomic Responses to Chronic Stress and Ketamine.Molecular pharmacology · 2024Article
- The integrated stress response effector GADD34 is repurposed by neurons to promote stimulus-induced translation.Cell reports · 2024Article
- Distinct hypoxia-induced translational profiles of embryonic and adult-derived macrophages.iScience · 2023Article
- Mu Opioid Receptor-Positive Neurons in the Dorsal Raphe Nucleus Are Impaired by Morphine Abstinence.Biological psychiatry · 2023Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
Translational profiling methodologies enable the systematic characterization of cell types in complex tissues, such as the mammalian brain, where neuronal isolation is exceptionally difficult. Here, we report a versatile strategy for profiling CNS cell types in a spatiotemporally restricted fashion by engineering a Cre-dependent adeno-associated virus expressing an EGFP-tagged ribosomal protein (AAV-FLEX-EGFPL10a) to access translating mRNAs by translating ribosome affinity purification (TRAP). We demonstrate the utility of this AAV to target a variety of genetically and anatomically defined neural populations expressing Cre recombinase and illustrate the ability of this viral TRAP (vTRAP) approach to recapitulate the molecular profiles obtained by bacTRAP in corticothalamic neurons across multiple serotypes. Furthermore, spatially restricting adeno-associated virus (AAV) injections enabled the elucidation of regional differences in gene expression within this cell type. Altogether, these results establish the broad applicability of the vTRAP strategy for the molecular dissection of any CNS or peripheral cell type that can be engineered to express Cre.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.