ArticlePloS one2017
Comprehensive analysis of phospholipids and glycolipids in the opportunistic pathogen Enterococcus faecalis.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 84 citations in OpenAlex.
- The DUF998 family protein DrmA modulates cationic glycolipid levels and the emergence of high-level daptomycin resistance inbioRxiv : the preprint server for biology · 2026Article
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- A two-component system signaling hub controls enterococcal membrane remodeling in response to daptomycin.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Genomic insights intoFrontiers in veterinary science · 2026Article
- Species-dependent susceptibility of enterococci to bovine cathelicidin BMAP-28 and its relationship to membrane fatty acid composition.Frontiers in microbiology · 2026Article
- Cyclodextrin derivatives that exhibitRSC medicinal chemistry · 2025Article
- Lysyl-Phosphatidylglycerol: A Lipid Involved in the Resistance ofAntibiotics (Basel, Switzerland) · 2025Review
- The Enteric Bacterium Enterococcus faecalis Elongates and Incorporates Exogenous Short and Medium Chain Fatty Acids Into Membrane Lipids.Molecular microbiology · 2024Article
- Lipidomic analyses reveal distinctive variations in homeoviscous adaptation among clinical strains ofMicrobiology spectrum · 2024Article
- Environmental Influence on Bacterial Lipid Composition: Insights from Pathogenic and Probiotic Strains.ACS omega · 2024Article
- The wound microbiota: microbial mechanisms of impaired wound healing and infection.Nature reviews. Microbiology · 2024Review
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- The Synthetic Peptide LyeTx I mn∆K, Derived fromAntibiotics (Basel, Switzerland) · 2024Article
- Profiling Antibiotic Susceptibility among DistinctAntibiotics (Basel, Switzerland) · 2023Article
- Alpha-defensins inhibit ERK/STAT3 signaling during monocyte-macrophage differentiation and impede macrophage function.Respiratory research · 2023Article
- Lipidomics Characterization of the Microbiome in People with Diabetic Foot Infection Using MALDI-TOF MS.Analytical chemistry · 2023Article
- The effects of daptomycin on cell wall biosynthesis in Enterococcal faecalis.Scientific reports · 2023Article
- Dual stable isotopes enhance lipidomic studies in bacterial model organism Enterococcus faecalis.Analytical and bioanalytical chemistry · 2023Article
- Too Much or Not Enough: The Role ofmBio · 2023Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterococcus faecalis is a Gram-positive, opportunistic, pathogenic bacterium that causes a significant number of antibiotic-resistant infections in hospitalized patients. The development of antibiotic resistance in hospital-associated pathogens is a formidable public health threat. In E. faecalis and other Gram-positive pathogens, correlations exist between lipid composition and antibiotic resistance. Resistance to the last-resort antibiotic daptomycin is accompanied by a decrease in phosphatidylglycerol (PG) levels, whereas multiple peptide resistance factor (MprF) converts anionic PG into cationic lysyl-PG via a trans-esterification reaction, providing resistance to cationic antimicrobial peptides. Unlike previous studies that relied on thin layer chromatography and spectrophotometry, we have performed liquid chromatography-tandem mass spectrometry (LC-MS/MS) directly on lipids extracted from E. faecalis, and quantified the phospholipids through multiple reaction monitoring (MRM). In the daptomycin-sensitive E. faecalis strain OG1RF, we have identified 17 PGs, 8 lysyl-PGs (LPGs), 23 cardiolipins (CL), 3 glycerophospho-diglucosyl-diacylglycerols (GPDGDAG), 5 diglucosyl-diacylglycerols (DGDAG), 3 diacylglycerols (DAGs), and 4 triacylglycerols (TAGs). We have quantified PG and shown that PG levels vary during growth of E. faecalis in vitro. We also show that two daptomycin-resistant (DapR) strains of E. faecalis have substantially lower levels of PG and LPG levels. Since LPG levels in these strains are lower, daptomycin resistance is likely due to the reduction in PG. This lipidome map is the first comprehensive analysis of membrane phospholipids and glycolipids in the important human pathogen E. faecalis, for which antimicrobial resistance and altered lipid homeostasis have been intimately linked.
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