ArticlePloS one2017
Transcriptome and proteome responses in RNAlater preserved tissue of Arabidopsis thaliana.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Long-term preservation strategy for legume root nodule phenotypes coupled with a comprehensive evaluation method.BMC plant biology · 2026Article
- Assessment of different preservation techniques for human skeletal muscle biopsy samples: A comparative method study on freeze-drying, RNAlater, and RNAlater-ICE.Physiological reports · 2025Article
- Rotifers in space: transcriptomic response of the bdelloid rotifer Adineta vaga aboard the International Space Station.BMC biology · 2025Article
- Effect of fieldwork-friendly coffee blender-based extraction methods and leaf tissue storage on the transcriptome of non-model plants.Journal of plant research · 2025Article
- Your Blood is Out for Delivery: Considerations of Shipping Time and Temperature on Degradation of RNA from Stabilized Whole Blood.Analytical chemistry · 2025Article
- Laser capture microdissection.Nature reviews. Methods primers · 2025Article
- Development of an infiltration-based RNA preservation method for cryogen-free storage of leaves for gene expression analyses in field-grown plants.Plant methods · 2024Article
- Optimal liquid-based DNA preservation for DNA barcoding of field-collected fungal specimens.Heliyon · 2024Article
- Your Blood is Out for Delivery: Considerations of Shipping Time and Temperature on Degradation of RNA from Stabilized Whole Blood.bioRxiv : the preprint server for biology · 2024Article
- Drying as an effective method to store soil samples for DNA-based microbial community analyses: a comparative study.Scientific reports · 2024Article
- Article
- A Comparative Study on the Effect of Euthanasia Methods and Sample Storage Conditions on RNA Yield and Quality in Porcine Tissues.Animals : an open access journal from MDPI · 2023Article
- Integrative transcriptomics and proteomics profiling ofFrontiers in plant science · 2023Article
- Better late than never: Optimising the proteomic analysis of field-collected octopus.PloS one · 2023Article
- Challenges and considerations for single-cell and spatially resolved transcriptomics sample collection during spaceflight.Cell reports methods · 2022Review
- Recent transcriptomic studies to elucidate the plant adaptive response to spaceflight and to simulated space environments.iScience · 2022Review
- Preserving plant samples from remote locations for detection of RNA and DNA viruses.Frontiers in microbiology · 2022Article
- Detection of Genes inGenes · 2021Article
- A New Era for Space Life Science: International Standards for Space Omics Processing.Patterns (New York, N.Y.) · 2020Review
- Article
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Authors and funding
4 authors.
Funding
Abstract
Tissue preservation is a minimal requirement for the success of plant RNA and protein expression studies. The standard of snap-freezing in liquid nitrogen is not always practical or possible. RNAlater, a concentrated solution of ammonium and cesium sulfates, has become a standard preservative in the absence of liquid nitrogen. Here, we demonstrate the effectiveness of RNAlater in preserving both RNA and proteins in Arabidopsis thaliana tissues for use in RNAseq and LC-MS/MS analysis of proteins. While successful in preserving plant material, a transcriptomic and proteomic response is evident. Specifically, 5770 gene transcripts, 84 soluble proteins, and 120 membrane-bound proteins were found to be differentially expressed at a log-fold change of ±1 (P ≤ 0.05). This response is mirrored in the abundance of post-translational modifications, with 23 of the 108 (21.3%) phosphorylated proteins showing altered abundance at a log-fold change of ±1 (P ≤ 0.05). While RNAlater is effective in preserving biological information, our findings warrant caution in its use for transcriptomic and proteomic experiments.
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