Trial reportAmerican journal of respiratory and critical care medicine2017
Vitamin C Prevents Offspring DNA Methylation Changes Associated with Maternal Smoking in Pregnancy.
Trial report in American journal of respiratory and critical care medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00632476 (In-Utero Smoke, Vitamin C, and Newborn Lung Function), which is not on this map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
In-Utero Smoke, Vitamin C, and Newborn Lung Function
Who cites it
42 citing papers in PubMed, 97 citations in OpenAlex.
- Improvements in lung function following vitamin C supplementation to pregnant smokers are associated with buccal DNA methylation at 5 years of age.Clinical epigenetics · 2024Trial
- Nicotine Exposure During Gestation: From Placental Dysfunction to Long-Term Neurobehavioral Outcomes.Birth defects research · 2026Review
- Maternal and perinatal nutritional programming of lung health and disease in childhood and early adulthood: Research gaps and opportunities-National Heart, Lung, and Blood Institute/National Institutes of Health Workshop Report.The Journal of allergy and clinical immunology · 2026Article
- Echoes of smoke: unwinding multigenerational asthma risk through longitudinal cohorts.ERJ open research · 2026Article
- Daily intake of antioxidants ameliorates PMFrontiers in nutrition · 2026Article
- Vitamin C in Pregnancy: A Key Modulator of Fetal Growth and Epigenetic Stability.Current nutrition reports · 2025Review
- Vitamin C-Dependent Intergenerational Inheritance of Enhanced Endurance Performance Following Maternal Exercise.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Impact of E-Cigarettes on Fetal and Neonatal Lung Development: The Influence of Oxidative Stress and Inflammation.Antioxidants (Basel, Switzerland) · 2025Review
- Comprehensive evaluation of smoking exposures and their interactions on DNA methylation.EBioMedicine · 2024Article
- Electroacupuncture may protect pulmonary dysplasia in offspring with perinatal nicotine exposure by altering maternal gut microbiota and metabolites.Frontiers in microbiology · 2024Article
- Contrasting Association of Maternal Plasma Biomarkers of Smoking and 1-Carbon Micronutrients with Offspring DNA Methylation: Evidence of Aryl Hydrocarbon Receptor Repressor Gene-Smoking-Folate Interaction.The Journal of nutrition · 2023Article
- The Role of Vitamins in the Pathogenesis of Asthma.International journal of molecular sciences · 2023Review
- DNA-Methylation Signatures of Tobacco Smoking in a High Cardiovascular Risk Population: Modulation by the Mediterranean Diet.International journal of environmental research and public health · 2023Article
- Dietary Supplementation Practices Among Undergraduate Students in Greece During the COVID-19 Pandemic and Their Association with COVID-19-related Anxiety.Materia socio-medica · 2023Article
- The methylation profile ofFrontiers in immunology · 2023Article
- Epigenetic regulation of immune function in asthma.The Journal of allergy and clinical immunology · 2022Review
- Impact of vitamin C supplementation on placental DNA methylation changes related to maternal smoking: association with gene expression and respiratory outcomes.Clinical epigenetics · 2021Article
- DNA methylome perturbations: an epigenetic basis for the emergingly heritable neurodevelopmental abnormalities associated with maternal smoking and maternal nicotine exposure†.Biology of reproduction · 2021Review
- Fetal Programming: Lung Health and Disease.Turkish thoracic journal · 2021Article
- Vitamin C Cytotoxicity and Its Effects in Redox Homeostasis and Energetic Metabolism in Papillary Thyroid Carcinoma Cell Lines.Antioxidants (Basel, Switzerland) · 2021Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
rationaleInfants whose mothers smoked during pregnancy demonstrate lifelong decreases in pulmonary function. DNA methylation changes associated with maternal smoking during pregnancy have been described in placenta and cord blood at delivery, in fetal lung, and in buccal epithelium and blood during childhood. We demonstrated in a randomized clinical trial ( ClinicalTrials.gov identifier, NCT00632476) that vitamin C supplementation to pregnant smokers can lessen the impact of maternal smoking on offspring pulmonary function and decrease the incidence of wheeze at 1 year of age.
objectivesTo determine whether vitamin C supplementation reduces changes in offspring methylation in response to maternal smoking and whether methylation at specific CpGs is also associated with respiratory outcomes.
methodsTargeted bisulfite sequencing was performed with a subset of placentas, cord blood samples, and buccal samples collected during the NCT00632476 trial followed by independent validation of selected cord blood differentially methylated regions, using bisulfite amplicon sequencing. MEASUREMENTS AND MAIN
resultsThe majority (69.03%) of CpGs with at least 10% methylation difference between placebo and nonsmoker groups were restored (by at least 50%) toward nonsmoker levels with vitamin C treatment. A significant proportion of restored CpGs were associated with phenotypic outcome with greater enrichment among hypomethylated CpGs.
conclusionsWe identified a pattern of normalization in DNA methylation by vitamin C supplementation across multiple loci. The consistency of this pattern across tissues and time suggests a systemic and persistent effect on offspring DNA methylation. Further work is necessary to determine how genome-wide changes in DNA methylation may mediate or reflect persistent effects of maternal smoking on lung function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.