ArticleNanoscale2017
3D single-molecule tracking enables direct hybridization kinetics measurement in solution.
Article in Nanoscale, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Active-Feedback 3D Single-Molecule Tracking Using a Fast-Responding Galvo Scanning Mirror.The journal of physical chemistry. A · 2023Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Generative adversarial network enables rapid and robust fluorescence lifetime image analysis in live cells.Communications biology · 2022Article
- Determining Sequence-Dependent DNA Oligonucleotide Hybridization and Dehybridization Mechanisms Using Coarse-Grained Molecular Simulation, Markov State Models, and Infrared Spectroscopy.Journal of the American Chemical Society · 2021Article
- Recent developments in the characterization of nucleic acid hybridization kinetics.Current opinion in biomedical engineering · 2021Article
- Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy.Entropy (Basel, Switzerland) · 2021Article
- DNA hybridisation kinetics using single-molecule fluorescence imaging.Essays in biochemistry · 2021Review
- Article
- Real-time 3D single molecule tracking.Nature communications · 2020Article
- Measuring DNA Hybridization Kinetics in Live Cells Using a Time-Resolved 3D Single-Molecule Tracking Method.Journal of the American Chemical Society · 2019Article
- An Adaptive Real-Time 3D Single Particle Tracking Method for Monitoring Viral First Contacts.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells.Molecules (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Single-molecule measurements of DNA hybridization kinetics are mostly performed on a surface or inside a trap. Here we demonstrate a time-resolved, 3D single-molecule tracking (3D-SMT) method that allows us to follow a freely diffusing ssDNA molecule in solution for hundreds of milliseconds or even seconds and observe multiple annealing and melting events taking place on the same molecule. This is achieved by combining confocal-feedback 3D-SMT with time-domain fluorescence lifetime measurement, where fluorescence lifetime serves as the indicator of hybridization. With sub-diffraction-limit spatial resolution in molecular tracking and 15 ms temporal resolution in monitoring the change of reporter's lifetime, we have demonstrated a full characterization of annealing rate (k
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.