Evidence map›Paper›PMID 28420692›Full record

ArticleGenome research2017

An improved assembly and annotation of the allohexaploid wheat genome identifies complete families of agronomic genes and provides genomic evidence for chromosomal translocations.

Bernardo J Clavijo, Luca Venturini, Christian Schudoma, Gonzalo Garcia Accinelli, Gemy Kaithakottil, Jonathan Wright, Philippa Borrill, George Kettleborough, Darren Heavens, Helen Chapman and 29 more

Abstract read
In one paragraph

Article in Genome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 212 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
212citing papers in PubMed, 4 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

212 citing papers in PubMed, 4 syntheses or guidelines pooled it.

  1. Pooled it
  2. Meta-QTLs, ortho-meta-QTLs and candidate genes for grain yield and associated traits in wheat (Triticum aestivum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022
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  13. The Role ofInternational journal of molecular sciences · 2025
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  20. Wheat NAC transcription factorPlant direct · 2024
    Article

152 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

39 authors.

Bernardo J ClavijoEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0002-7597-2774
Luca VenturiniEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0001-7824-1507
Christian SchudomaEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0003-1157-1354
Gonzalo Garcia AccinelliEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Gemy KaithakottilEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0003-1360-7808
Jonathan WrightEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Philippa BorrillJohn Innes Centre, Norwich, NR4 7UH, United Kingdom.ORCID 0000-0002-7623-8256
George KettleboroughEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Darren HeavensEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Helen ChapmanEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
James LipscombeEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Tom BarkerEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Fu-Hao LuJohn Innes Centre, Norwich, NR4 7UH, United Kingdom.ORCID 0000-0003-3783-4685
Neil McKenzieJohn Innes Centre, Norwich, NR4 7UH, United Kingdom.
Dina RaatsEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0002-1046-3346
Ricardo H Ramirez-GonzalezEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0001-5745-7085
Aurore CoinceEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Ned PeelEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Lawrence Percival-AlwynEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0001-7725-9203
Owen DuncanARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley Western Australia 6009, Australia.ORCID 0000-0001-6999-1509
Josua TröschARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley Western Australia 6009, Australia.ORCID 0000-0002-7770-7333
Guotai YuJohn Innes Centre, Norwich, NR4 7UH, United Kingdom.
Dan M BolserEMBL European Bioinformatics Institute, Hinxton, CB10 1SD, United Kingdom.ORCID 0000-0002-3991-0859
Guy NamaatiEMBL European Bioinformatics Institute, Hinxton, CB10 1SD, United Kingdom.ORCID 0000-0002-0523-4071
Arnaud KerhornouEMBL European Bioinformatics Institute, Hinxton, CB10 1SD, United Kingdom.ORCID 0000-0002-2890-295X
Manuel SpannaglPlant Genome and Systems Biology, Helmholtz Center Munich, 85764 Neuherberg, Germany.
Heidrun GundlachPlant Genome and Systems Biology, Helmholtz Center Munich, 85764 Neuherberg, Germany.ORCID 0000-0002-6757-0943
Georg HabererPlant Genome and Systems Biology, Helmholtz Center Munich, 85764 Neuherberg, Germany.
Robert P DaveyEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0002-5589-7754
Christine FoskerEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0002-3799-9474
Federica Di PalmaEarlham Institute, Norwich, NR4 7UZ, United Kingdom.
Andrew L PhillipsRothamsted Research, Harpenden, AL5 2JQ, United Kingdom.ORCID 0000-0002-6406-7015
A Harvey MillarARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley Western Australia 6009, Australia.ORCID 0000-0001-9679-1473
Paul J KerseyEMBL European Bioinformatics Institute, Hinxton, CB10 1SD, United Kingdom.ORCID 0000-0002-7054-800X
Cristobal UauyJohn Innes Centre, Norwich, NR4 7UH, United Kingdom.ORCID 0000-0002-9814-1770
Ksenia V KrasilevaEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0002-1679-0700
David SwarbreckEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0002-5453-1013
Michael W BevanJohn Innes Centre, Norwich, NR4 7UH, United Kingdom.ORCID 0000-0001-8264-2354
Matthew D ClarkEarlham Institute, Norwich, NR4 7UZ, United Kingdom.ORCID 0000-0002-8049-5423

Funding

Biotechnology and Biological Sciences Research Council BB/J00328X/1Biotechnology and Biological Sciences Research Council BB/J003557/1Biotechnology and Biological Sciences Research Council BB/J003743/1Biotechnology and Biological Sciences Research Council BB/J003913/1Biotechnology and Biological Sciences Research Council BB/J004588/1Biotechnology and Biological Sciences Research Council BB/J004669/1Biotechnology and Biological Sciences Research Council BB/J010375/1Biotechnology and Biological Sciences Research Council BBS/E/C/00005202Biotechnology and Biological Sciences Research Council BBS/E/T/000PR9817
6 · The paper itself

Abstract

Advances in genome sequencing and assembly technologies are generating many high-quality genome sequences, but assemblies of large, repeat-rich polyploid genomes, such as that of bread wheat, remain fragmented and incomplete. We have generated a new wheat whole-genome shotgun sequence assembly using a combination of optimized data types and an assembly algorithm designed to deal with large and complex genomes. The new assembly represents >78% of the genome with a scaffold N50 of 88.8 kb that has a high fidelity to the input data. Our new annotation combines strand-specific Illumina RNA-seq and Pacific Biosciences (PacBio) full-length cDNAs to identify 104,091 high-confidence protein-coding genes and 10,156 noncoding RNA genes. We confirmed three known and identified one novel genome rearrangements. Our approach enables the rapid and scalable assembly of wheat genomes, the identification of structural variants, and the definition of complete gene models, all powerful resources for trait analysis and breeding of this key global crop.

Indexed as

Genome, PlantTranslocation, GeneticAlgorithmsContig MappingMolecular Sequence AnnotationPlant ProteinsPolymorphism, GeneticPolyploidyTriticumPlant Proteins

Identifiers

PMID28420692
PMCPMC5411782

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.