ArticleStem cell research & therapy2017
Sialylation regulates myofibroblast differentiation of human skin fibroblasts.
Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Mammalian Neuraminidases in Immune-Mediated Diseases: Mucins and Beyond.Frontiers in immunology · 2022Pooled it
- N6-methyladenosine modification of NEU1 mediated by METTL3 exacerbates angiotensin II-induced atrial fibrillation.Cell adhesion & migration · 2026Article
- Sustained-Release Sitagliptin Microneedles for Scar Prevention via Fibroblast-to-Adipocyte Conversion.Small science · 2025Article
- CD44 as a Central Integrator of Inflammation and Fibrosis: From Molecular Signaling to Environmental Modulation.International journal of molecular sciences · 2025Review
- Comparative analysis of antioxidant activity and accelerated collagen expression of human skin fibroblasts with gelatin hydrolysates from different sources.Food chemistry: X · 2025Article
- Article
- Glycan Modifications as Regulators of Stem Cell Fate.Biology · 2024Review
- Spatiotemporal changes of tissue glycans depending on localization in cardiac aging.Regenerative therapy · 2023Article
- Article
- Sialidase Inhibitors with Different Mechanisms.Journal of medicinal chemistry · 2022Review
- High-dimensionality reduction clustering of complex carbohydrates to study lung cancer metabolic heterogeneity.Advances in cancer research · 2022Article
- Glycan characteristics of human heart constituent cells maintaining organ function: relatively stable glycan profiles in cellular senescence.Biogerontology · 2021Article
- Glycosylation and Aging.Advances in experimental medicine and biology · 2021Article
- Glycome profiling by lectin microarray reveals dynamic glycan alterations during epidermal stem cell aging.Aging cell · 2020Article
- Glycosylation of proteins of human skin fibroblasts is changed by rosmarinic acid.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in FibroblastsBioMed research international · 2020Article
- Vascular Diseases and Gangliosides.International journal of molecular sciences · 2019Review
- Establishment and characterization of a novel neuroendocrine carcinoma cell line derived from a human ascending colon tumor.Cancer science · 2019Article
- Increase of MAL-II Binding Alpha2,3-Sialylated Glycan Is Associated with 5-FU Resistance and Short Survival of Cholangiocarcinoma Patients.Medicina (Kaunas, Lithuania) · 2019Article
- Development and characterization of a stable bovine intestinal sub-epithelial myofibroblast cell line from ileum of a young calf.In vitro cellular & developmental biology. Animal · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFibroblasts are key players in maintaining skin homeostasis and in orchestrating physiological tissue repair and skin regeneration. Dysfunctions in fibroblasts that occur with aging and the senescent process lead to the delayed healing observed in elderly people. The molecular mechanisms leading to fibroblast dysfunction during aging and the senescent process have not yet been clarified. Previously, changes in patterns of glycosylation were observed in fibroblasts in aging and the senescent process, but the effect of these changes on the function of fibroblasts has not been well documented. Here, we investigated whether changes in glycosylation during the process to senescence may have functional effects on fibroblasts.
methodsThe changes in cell surface glycans on skin fibroblasts during the process to senescence were examined in early-passage (EP) and late-passage (LP) skin fibroblasts by fluorescence-activated cell sorting analysis using lectins. The contributors to the changes in cell surface glycans were examined by real-time polymerase chain reaction or Western blot analysis. The effects of changes in glycosylation on proliferation, migration, induction of cellular senescence, and myofibroblast differentiation induced by transforming growth factor (TGF)-β1 stimulation were examined in EP fibroblasts. The changes in glycosylation were performed by GalNAc-α-O-benzyl or sialidase treatment.
resultsA decrease in sialylation of glycoproteins and an increase in sialidase NEU1 were observed in LP fibroblasts. The reduction of sialylation did not have any effect on proliferation, migration, or induction of cellular senescence. On the other hand, myofibroblast differentiation was inhibited by the reduction of sialylation, indicating that sialylation is important for myofibroblast differentiation. The localization of CD44 in lipid rafts, which is required for myofibroblast differentiation, was inhibited by the reduction of sialylation. Furthermore, reduced myofibroblast differentiation in LP fibroblasts was restored by a sialidase inhibitor.
conclusionsDesialylation of CD44 with increased sialidase during the process to senescence reduced the localization of CD44 in lipid rafts after TGF-β1 stimulation, leading to the inhibition of myofibroblast differentiation. Thus, regulation of sialylation may be an attractive strategy for the prevention and regenerative therapy of age-related skin diseases, cosmetic skin alterations, and chronic wounds caused by delayed healing in elderly people.
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