ArticleJournal of biophotonics2017
Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies.
Article in Journal of biophotonics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Label-free 4D holotomography with depth-adaptive segmentation for quantitative analysis of lipid droplet dynamics in hepatic organoids.Biophotonics discovery · 2026Article
- Influence of sample-specific properties on light scattering by human milk.Biophotonics discovery · 2026Article
- Disordered Glass Nanowire Substrates Produce in Vivo-Like Astrocyte Morphology Revealed by Low-Coherence Holotomography.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Label-free refractive index mapping of human sperm cells.Biomedical optics express · 2025Article
- Structural-Scaling Transitions and Criticality Cascade in DNA with Open States.International journal of molecular sciences · 2025Article
- Dissecting Morphological and Functional Dynamics of Non-Tumorigenic and Triple-Negative Breast Cancer Cell Lines Using PCA and t-SNE Analysis.Cancer reports (Hoboken, N.J.) · 2025Article
- VS-FPM: Large-Format, Label-Free Virtual Histopathology Microscopy.BME frontiers · 2025Article
- Finite element modeling of plasmonic resonances in photothermal gold nanoparticles embedded in cells.Nanoscale advances · 2024Article
- Multimodal mechano-microscopy reveals mechanical phenotypes of breast cancer spheroids in three dimensions.APL bioengineering · 2024Article
- Photonic Artifacts in Ratiometric Luminescence Nanothermometry.Nano letters · 2023Article
- Comparing the segmentation of quantitative phase images of neurons using convolutional neural networks trained on simulated and augmented imagery.Neurophotonics · 2023Article
- Rapid and precise tracking of water influx and efflux across cell membranes induced by a pulsed electric field.Biomedical optics express · 2023Article
- Immature and mature bone marrow-derived dendritic cells exhibit distinct intracellular mechanical properties.Scientific reports · 2023Article
- Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing.Scientific reports · 2022Article
- Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy.Scientific reports · 2022Article
- Review
- Fluorescence Microscopy-An Outline of Hardware, Biological Handling, and Fluorophore Considerations.Cells · 2021Review
- Label-Free and Quantitative Dry Mass Monitoring for Single Cells during In Situ Culture.Cells · 2021Article
- Assessment of internal refractive index profile of stochastically inhomogeneous nuclear models via analysis of two-dimensional optical scattering patterns.Journal of biomedical optics · 2021Article
- Reconstruction of angle-resolved backscattering through a multimode fiber for cell nuclei and particle size determination.APL photonics · 2020Article
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Abstract
The refractive index (RI) of biological materials is a fundamental parameter for the optical characterization of living systems. Numerous light scattering technologies are grounded in a quantitative knowledge of the refractive index at cellular and subcellular scales. Recent work in quantitative phase microscopy (QPM) has called into question the widely held assumption that the index of the cell nucleus is greater than that of the cytoplasm, a result which disagrees with much of the current literature. In this work, we critically examine the measurement of the nuclear and whole-cell refractive index using QPM, validating that nuclear refractive index is lower than that of cytoplasm in four diverse cell lines and their corresponding isolated nuclei. We further examine Mie scattering and phase-wrapping as potential sources of error in these measurements, finding they have minimal impact. Finally, we use simulation to examine the effects of incorrect RI assumptions on nuclear morphology measurements using angle-resolved scattering information. Despite an erroneous assumption of the nuclear refractive index, accurate measurement of nuclear morphology was maintained, suggesting that light scattering modalities remain effective.
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