ArticleScientific reports2017
Live imaging of H3K9 acetylation in plant cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Real-time single-molecule imaging of transcriptional regulatory networks in living cells.Nature reviews. Genetics · 2024Review
- Rice responds to Spodoptera frugiperda infestation via epigenetic regulation of H3K9ac in the jasmonic acid signaling and phenylpropanoid biosynthesis pathways.Plant cell reports · 2024Article
- Development of Chromatin Immunoprecipitation for the Analysis of Histone Modifications in Red Macroalga Neopyropia yezoensis (Rhodophyta).Molecular biotechnology · 2023Article
- Histone deacetylation regulates de novo shoot regeneration.PNAS nexus · 2023Article
- N-acetylglutamic acid alleviates oxidative stress based on histone acetylation in plants.Frontiers in plant science · 2023Article
- Studying Chromatin Epigenetics with Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Live-cell imaging probes to track chromatin modification dynamics.Microscopy (Oxford, England) · 2021Article
- Genome Maintenance Mechanisms at the Chromatin Level.International journal of molecular sciences · 2021Review
- A live imaging system to analyze spatiotemporal dynamics of RNA polymerase II modification in Arabidopsis thaliana.Communications biology · 2021Article
- The Histone Marks Signature in Exonic and Intronic Regions Is Relevant in Early Response of Tomato Genes toPlants (Basel, Switzerland) · 2020Article
- Histone Deacetylase Is Required for GA-Induced Programmed Cell Death in Maize Aleurone Layers.Plant physiology · 2017Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proper regulation of histone acetylation is important in development and cellular responses to environmental stimuli. However, the dynamics of histone acetylation at the single-cell level remains poorly understood. Here we established a transgenic plant cell line to track histone H3 lysine 9 acetylation (H3K9ac) with a modification-specific intracellular antibody (mintbody). The H3K9ac-specific mintbody fused to the enhanced green fluorescent protein (H3K9ac-mintbody-GFP) was introduced into tobacco BY-2 cells. We successfully demonstrated that H3K9ac-mintbody-GFP interacted with H3K9ac in vivo. The ratio of nuclear/cytoplasmic H3K9ac-mintbody-GFP detected in quantitative analysis reflected the endogenous H3K9ac levels. Under chemically induced hyperacetylation conditions with histone deacetylase inhibitors including trichostatin A, Ky-2 and Ky-14, significant enhancement of H3K9ac was detected by H3K9ac-mintbody-GFP dependent on the strength of inhibitors. Conversely, treatment with a histone acetyltransferase inhibitor, C646 caused a reduction in the nuclear to cytoplasmic ratio of H3K9ac-mintbody-GFP. Using this system, we assessed the environmental responses of H3K9ac and found that cold and salt stresses enhanced H3K9ac in tobacco BY-2 cells. In addition, a combination of H3K9ac-mintbody-GFP with 5-ethynyl-2'-deoxyuridine labelling confirmed that H3K9ac level is constant during interphase.
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Registered trials
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