ArticleProceedings of the National Academy of Sciences of the United States of America2017
Effect of cholesterol on the molecular structure and transitions in a clinical-grade lung surfactant extract.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Cholesterol Regulates Airway Epithelial Cell Differentiation by Inhibiting p53 Nuclear Translocation.International journal of molecular sciences · 2025Article
- The role of antibiotic-derived mycobacterial vesicles in tuberculosis pathogenesis.Scientific reports · 2024Article
- RSK2-mediated cGAS phosphorylation induces cGAS chromatin-incorporation-mediated cell transformation and cancer cell colony growth.Cell death discovery · 2024Article
- Phospholipid Membrane Interactions of Model Ac-WL-X-LL-OH Peptides Investigated by Solid-State Nuclear Magnetic Resonance.Membranes · 2024Article
- Molecular Mobility in Keratin-Rich Materials Monitored by Nuclear Magnetic Resonance: A Tool for the Evaluation of Structure-Giving Properties.Biomacromolecules · 2023Article
- Time-domain proton-detected local-field NMR for molecular structure determination in complex lipid membranes.Magnetic resonance (Gottingen, Germany) · 2023Article
- Interfacial structure of pulmonary surfactants revisited: Cholesterol and surface pressure effects.Biophysical journal · 2022Article
- Effects of cholesterol on the structure and collapse of DPPC monolayers.Biophysical journal · 2022Article
- Rotational decoupling between the hydrophilic and hydrophobic regions in lipid membranes.Biophysical journal · 2022Article
- The role of high cholesterol in age-related COVID19 lethality.bioRxiv : the preprint server for biology · 2021Article
- Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs.Biomedicines · 2021Article
- The Potential Role of Bioactive Plasmalogens in Lung Surfactant.Frontiers in cell and developmental biology · 2021Review
- Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways-a European population-based cohort study.BMJ open respiratory research · 2021Article
- Excipient Enhanced Growth Aerosol Surfactant Replacement Therapy in anJournal of aerosol medicine and pulmonary drug delivery · 2020Article
- Structural Changes in Films of Pulmonary Surfactant Induced by Surfactant Vesicles.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Membrane Heterogeneity Beyond the Plasma Membrane.Frontiers in cell and developmental biology · 2020Review
- Self-Assembly in Ganglioside‒Phospholipid Systems: The Co-Existence of Vesicles, Micelles, and Discs.International journal of molecular sciences · 2019Article
- An Inward-Rectifier Potassium Channel Coordinates the Properties of Biologically Derived Membranes.Biophysical journal · 2019Article
- The Evolution of Cholesterol-Rich Membrane in Oxygen Adaption: The Respiratory System as a Model.Frontiers in physiology · 2019Review
- The Impact of Nonequilibrium Conditions in Lung Surfactant: Structure and Composition Gradients in Multilamellar Films.ACS central science · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lipid-protein film covering the interface of the lung alveolar in mammals is vital for proper lung function and its deficiency is related to a range of diseases. Here we present a molecular-level characterization of a clinical-grade porcine lung surfactant extract using a multitechnique approach consisting of [Formula: see text]-[Formula: see text] solid-state nuclear magnetic spectroscopy, small- and wide-angle X-ray scattering, and mass spectrometry. The detailed characterization presented for reconstituted membranes of a lung extract demonstrates that the molecular structure of lung surfactant strongly depends on the concentration of cholesterol. If cholesterol makes up about 11% of the total dry weight of lung surfactant, the surfactant extract adopts a single liquid-ordered lamellar phase, [Formula: see text], at physiological temperatures. This [Formula: see text] phase gradually changes into a liquid-disordered lamellar phase, [Formula: see text], when the temperature is increased by a few degrees. In the absence of cholesterol the system segregates into one lamellar gel phase and one [Formula: see text] phase. Remarkably, it was possible to measure a large set of order parameter magnitudes [Formula: see text] from the liquid-disordered and -ordered lamellar phases and assign them to specific C-H bonds of the phospholipids in the biological extract with no use of isotopic labeling. These findings with molecular details on lung surfactant mixtures together with the presented NMR methodology may guide further development of pulmonary surfactant pharmaceuticals that better mimic the physiological self-assembly compositions for treatment of pathological states such as respiratory distress syndrome.
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