Evidence map›Paper›PMID 28415766›Full record

ArticleOncotarget2017

Anti-proliferative role of recombinant lethal toxin of Bacillus anthracis on primary mammary ductal carcinoma cells revealing its therapeutic potential.

Rekha Khandia, Bramhadev Pattnaik, Katherukamem Rajukumar, Atul Pateriya, Sandeep Bhatia, Harshad Murugkar, Anil Prakash, Hare Krishna Pradhan, Kuldeep Dhama, Ashok Munjal and 1 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Rekha KhandiaICAR-National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.
Bramhadev PattnaikProject Directorate on Foot and Mouth Disease, Mukteswar, Uttarakhand, India.
Katherukamem RajukumarICAR-National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.
Atul PateriyaICAR-National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.
Sandeep BhatiaICAR-National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.
Harshad MurugkarICAR-National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.
Anil PrakashDepartment of Microbiology, Barkatullah University, Bhopal, Madhya Pradesh, India.
Hare Krishna PradhanEx-Avian Influenza National Consultant, Indian Office of WHO Consultant, Bhartiya Kala Kendra, New Delhi, India.
Kuldeep DhamaDivision of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly Uttar Pradesh, India.
Ashok MunjalDepartment of Biochemistry and Genetics, Barkatullah University, Bhopal, Madhya Pradesh, India.
Sunil K JoshiCellular Immunology Laboratory, Frank Reidy Research Center of Bioelectrics, College of Health Sciences, Old Dominion University Norfolk, VA USA.
National Institute of High Security Animal Diseases · INBarkatullah University · INIndian Veterinary Research Institute · INOld Dominion University · USProject Directorate on Foot and Mouth Disease · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacillus anthracis secretes three secretary proteins; lethal factor (LF), protective antigen (PA) and edema factor (EF). The LF has ability to check proliferation of mammary tumors, chiefly depending on mitogen activated protein kinase (MAPK) signaling pathway. Evaluation of therapeutic potential of recombinant LF (rLF), recombinant PA (rPA) and lethal toxin (rLF + rPA = LeTx) on the primary mammary ductal carcinoma cells revealed significant (p < 0.01) reduction in proliferation of tumor cells with mean inhibition indices of 28.0 ± 1.37% and 19.6 ± 1.47% respectively. However, treatment with rPA alone had no significant anti-proliferative effect as evident by low mean inhibition index of 3.4 ± 3.87%. The higher inhibition index observed for rLF alone as compared to LeTx is contrary to the existing knowledge on LF, which explains the requirement of PA dependent endocytosis for its enzymatic activity. Therefore, the plausible existence of PA independent mode of action of LF including direct receptor mediated endocytosis or modulation of signal transduction cascade via unknown means is hypothesized. In silico protein docking analysis of other cellular receptors for any plausibility to play the role of receptor for LF revealed c-Met receptor showing strongest affinity for LF (H bond = 19; Free energy = -773.96), followed by nerve growth factor receptor (NGFR) and human epidermal growth factor receptor (HER)-1. The study summarizes the use of rLF or LeTx as therapeutic molecule against primary mammary ductal carcinoma cells and also the c-Met as potential alternative receptor for LF to mediate and modulate PA independent signal transduction.

Indexed as

AgedAntigens, BacterialAntineoplastic AgentsBacterial ToxinsBreast NeoplasmsCarcinoma, Ductal, BreastCell Line, TumorCell ProliferationFemaleHumansMiddle AgedModels, MolecularMolecular ConformationNeoplasm StagingProtein BindingProto-Oncogene Proteins c-metanthrax toxinAntigens, BacterialAntineoplastic AgentsBacterial ToxinsProto-Oncogene Proteins c-metRecombinant Proteinsanthrax toxin receptorbacillus anthracisc-Met receptorlethal factorprotective antigen

Identifiers

PMID28415766
PMCPMC5482621
OpenAlexW2600335631

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.