Evidence map›Paper›PMID 28414312›Full record

ArticleNature cell biology2017

High-resolution myogenic lineage mapping by single-cell mass cytometry.

Ermelinda Porpiglia, Nikolay Samusik, Andrew Tri Van Ho, Benjamin D Cosgrove, Thach Mai, Kara L Davis, Astraea Jager, Garry P Nolan, Sean C Bendall, Wendy J Fantl and 1 more

Erratum issuedOpen access · greenAbstract read
In one paragraph

Article in Nature cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 75 papers.

0numbers the graph read from it
0cells of the map it votes in
75citing papers in PubMed
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

75 citing papers in PubMed, 130 citations in OpenAlex.

  1. A who's who of cell types in skeletal muscle.Communications biology · 2026
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  16. Satellite cell dynamics during skeletal muscle hypertrophy.Biochemical Society transactions · 2024
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15 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Ermelinda PorpigliaBlau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.ORCID 0000-0003-2652-899X
Nikolay SamusikBaxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Andrew Tri Van HoBlau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.
Benjamin D CosgroveBlau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.ORCID 0000-0003-2164-350X
Thach MaiBlau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.
Kara L DavisBaxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Astraea JagerBaxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Garry P NolanBaxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Sean C BendallBaxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Wendy J FantlBaxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Helen M BlauBlau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.
Stanford University · USCalifornia Institute for Regenerative Medicine · US

Funding

Stem Cells for Brain and BrawnR01AG020961 · NIA · STANFORD UNIVERSITY · PI BLAU, HELEN M · 2002 to 2019
$5.0M
Mass Cytometry Analysis of Signaling Dysfunction in Duchenne Muscular DystrophyR01NS089533 · NINDS · STANFORD UNIVERSITY · PI BLAU, HELEN M · 2014 to 2017
$1.3M
Supplement to: EX VIVO REJUVENATION AND EXPANSION OF MUSCLE STEM CELLS FROM AGED MICER00AG042491 · NIA · CORNELL UNIVERSITY · PI COSGROVE, BENJAMIN DAVID · 2014 to 2016
$790k
Ex vivo rejuvenation and expansion of muscle stem cells from aged miceK99AG042491 · NIA · STANFORD UNIVERSITY · PI COSGROVE, BENJAMIN DAVID · 2012 to 2013
$177k
NIA NIH HHS K99 AG042491NIA NIH HHS R00 AG042491NIA NIH HHS R01 AG020961NINDS NIH HHS R01 NS089533
6 · The paper itself

Abstract

Muscle regeneration is a dynamic process during which cell state and identity change over time. A major roadblock has been a lack of tools to resolve a myogenic progression in vivo. Here we capitalize on a transformative technology, single-cell mass cytometry (CyTOF), to identify in vivo skeletal muscle stem cell and previously unrecognized progenitor populations that precede differentiation. We discovered two cell surface markers, CD9 and CD104, whose combined expression enabled in vivo identification and prospective isolation of stem and progenitor cells. Data analysis using the X-shift algorithm paired with single-cell force-directed layout visualization defined a molecular signature of the activated stem cell state (CD44

Indexed as

Cell LineageMuscle DevelopmentRegenerationAnimalsBiomarkersCell ProliferationCells, CulturedCell SeparationElapid VenomsFlow CytometryFusion Regulatory Protein-1Genes, ReporterGenotypeHigh-Throughput Screening AssaysHyaluronan ReceptorsIntegrin beta4BiomarkersCd44 protein, mouseCd9 protein, mouseElapid VenomsFusion Regulatory Protein-1Hyaluronan ReceptorsIntegrin beta4Luminescent ProteinsMyoD1 myogenic differentiation proteinMyoD ProteinnotexinPax7 protein, mousePAX7 Transcription FactorTetraspanin 29

Identifiers

PMID28414312
PMCPMC5728993
OpenAlexW2606946599

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.