Evidence map›Paper›PMID 28414097›Full record

ReviewMethods (San Diego, Calif.)2017

Single-molecule and super-resolution imaging of transcription in living bacteria.

Mathew Stracy, Achillefs N Kapanidis

Abstract readReview
In one paragraph

Review in Methods (San Diego, Calif.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

  1. Article
  2. Review
  3. Kinetic Features of Degradation of R-Loops by RNase H1 fromInternational journal of molecular sciences · 2024
    Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. RNAP Promoter Search and Transcription Kinetics in LiveThe journal of physical chemistry. B · 2023
    Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Extraction of Hidden Science from Nanoscale Images.The journal of physical chemistry. C, Nanomaterials and interfaces · 2022
    Article
  15. Review
  16. Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021
    Review
  17. Article
  18. Revealing atomic-scale molecular diffusion of a plant-transcription factor WRKY domain protein along DNA.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Mathew StracyBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, United Kingdom. Electronic address: mathew.stracy@physics.ox.ac.uk.
Achillefs N KapanidisBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford OX1 3PU, United Kingdom. Electronic address: kapanidis@physics.ox.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/H01795X/1Biotechnology and Biological Sciences Research Council BB/J00054X/1Biotechnology and Biological Sciences Research Council BB/N018656/1European Research Council 261227Wellcome Trust 204684/Z/16/Z
6 · The paper itself

Abstract

In vivo single-molecule and super-resolution techniques are transforming our ability to study transcription as it takes place in its native environment in living cells. This review will detail the methods for imaging single molecules in cells, and the data-analysis tools which can be used to extract quantitative information on the spatial organization, mobility, and kinetics of the transcription machinery from these experiments. Furthermore, we will highlight studies which have applied these techniques to shed new light on bacterial transcription.

Indexed as

Transcription, GeneticBacterial ProteinsChromatin ImmunoprecipitationCrystallography, X-RayDNA, BacterialDNA-Directed RNA PolymerasesImage EnhancementImaging, Three-DimensionalKineticsLuminescent ProteinsMicroscopy, FluorescenceModels, BiologicalSingle Molecule ImagingBacterial ProteinsDNA, BacterialDNA-Directed RNA PolymerasesLuminescent ProteinsLive cellsPALMQuantitative microscopySingle-particle trackingSuper-resolution imagingTranscription

Identifiers

PMID28414097
PMCPMC5670121

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.