ArticleExperimental eye research2017
A mouse ocular explant model that enables the study of living optic nerve head events after acute and chronic intraocular pressure elevation: Focusing on retinal ganglion cell axons and mitochondria.
Article in Experimental eye research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Quantitative Flavoprotein Fluorescence Parameters in Retinal and Optic Nerve Diseases: A Scoping Review.Journal of clinical medicine · 2026Review
- Mitochondrial Transplantation in the Eye: A Review and Evaluation of Surgical Approaches.bioRxiv : the preprint server for biology · 2026Article
- Association between Nucleoside and Nucleotide Reverse Transcriptase Inhibitor Use and Primary Open-Angle Glaucoma Risk in All of Us.Ophthalmology · 2025Article
- Cellular responses to short-term intraocular pressure elevation in a porcine eye explant model.Experimental eye research · 2025Article
- Peripheral blood mononuclear cell respiratory function is associated with progressive glaucomatous vision loss.Nature medicine · 2024Article
- Review
- In vivo noninvasive mitochondrial redox assessment of the optic nerve head to predict disease.PNAS nexus · 2023Article
- Optic Nerve Injury Enhanced Mitochondrial Fission and Increased Mitochondrial Density without Altering the Uniform Mitochondrial Distribution in the Unmyelinated Axons of Retinal Ganglion Cells in a Mouse Model.International journal of molecular sciences · 2023Article
- Hydrogen sulfide supplement preserves mitochondrial function of retinal ganglion cell in a rat glaucoma model.Cell and tissue research · 2022Article
- The Role of Axonal Transport in Glaucoma.International journal of molecular sciences · 2022Review
- Effects of Iron and Zinc on Mitochondria: Potential Mechanisms of Glaucomatous Injury.Frontiers in cell and developmental biology · 2021Review
- Pressure-Induced Changes in Astrocyte GFAP, Actin, and Nuclear Morphology in Mouse Optic Nerve.Investigative ophthalmology & visual science · 2020Article
- A method to quantify regional axonal transport blockade at the optic nerve head after short term intraocular pressure elevation in mice.Experimental eye research · 2020Article
- The effects of age on mitochondria, axonal transport, and axonal degeneration after chronic IOP elevation using a murine ocular explant model.Experimental eye research · 2018Article
- Subtype-specific response of retinal ganglion cells to optic nerve crush.Cell death discovery · 2018Article
- Regional Retinal Ganglion Cell Axon Loss in a Murine Glaucoma Model.Investigative ophthalmology & visual science · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
We developed an explant model of the mouse eye and optic nerve that facilitates the study of retinal ganglion cell axons and mitochondria in the living optic nerve head (ONH) in an ex vivo environment. Two transgenic mouse strains were used, one expressing yellow fluorescent protein in selected axons and a second strain expressing cyan fluorescent protein in all mitochondria. We viewed an explanted mouse eye and optic nerve by laser scanning microscopy at and behind the ONH, the site of glaucoma injury. Explants from previously untreated mice were studied with the intraocular pressure (IOP) set artificially at normal or elevated levels for several hours. Explants were also studied from eyes that had undergone chronic IOP elevation from 14 h to 6 weeks prior to ex vivo study. Image analysis in static images and video of individual mitochondria or axonal structure determined effects of acute and chronic IOP elevation. At normal IOP, fluorescent axonal structure was stable for up to 3 h under ex vivo conditions. After chronic IOP elevation, axonal integrity index values indicated fragmentation of axon structure in the ONH. In mice with fluorescent mitochondria, the normal density decreased with distance behind the ONH by 45% (p = 0.002, t-test). Density increased with prior chronic IOP elevation to 21,300 ± 4176 mitochondria/mm
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Registered trials
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