ArticleEpigenetics & chromatin2017
Differential DNA methylation at conserved non-genic elements and evidence for transgenerational inheritance following developmental exposure to mono(2-ethylhexyl) phthalate and 5-azacytidine in zebrafish.
Article in Epigenetics & chromatin, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Sex-specific differences in zebrafish brains.Biology of sex differences · 2022Pooled it
- Neuroendocrine Disruption of Reproduction by Bisphenols and Phthalates: From Molecular Mechanisms to Reproductive Outcomes.International journal of molecular sciences · 2026Review
- Phthalate-induced effects in algae and fishes: insights into environmental toxicology.Environmental science and pollution research international · 2026Review
- Teleosts as Models for Epigenetic Inheritance.Molecular ecology · 2026Review
- Phthalate Esters in Aquatic Ecosystems: A Multiscale Threat from Molecular Disruption to Ecological Risks.Toxics · 2026Review
- Review
- Genome-wide DNA methylation of the liver reveals delayed effects of early-life exposure to 17-α-ethinylestradiol in the self-fertilizing mangrove rivulus.Epigenetics · 2022Article
- Emerging concepts and opportunities for endocrine disruptor screening of the non-EATS modalities.Environmental research · 2022Article
- Genetic and epigenetic regulation of growth, reproduction, disease resistance and stress responses in aquaculture.Frontiers in genetics · 2022Review
- Epigenetic Transgenerational Modifications Induced by Xenobiotic Exposure in Zebrafish.Frontiers in cell and developmental biology · 2022Review
- Review
- Associations among phthalate exposure, DNA methylation of TSLP, and childhood allergy.Clinical epigenetics · 2021Article
- Endocrine Disruptors Acting on Estrogen and Androgen Pathways Cause Reproductive Disorders through Multiple Mechanisms: A Review.International journal of environmental research and public health · 2021Review
- Article
- Intergenerational transfer of DNA methylation marks in the honey bee.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Developmental exposure window influences silver toxicity but does not affect the susceptibility to subsequent exposures in zebrafish embryos.Histochemistry and cell biology · 2020Article
- Dietary inclusion of plant ingredients induces epigenetic changes in the intestine of zebrafish.Epigenetics · 2020Article
- TGF-β1 relieves epithelial-mesenchymal transition reduction in hypospadias induced by DEHP in rats.Pediatric research · 2020Article
- Epigenetic, transcriptional and phenotypic responses in two generations ofEnvironmental epigenetics · 2019Article
- Functions and mechanisms of epigenetic inheritance in animals.Nature reviews. Molecular cell biology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExposure to environmental stressors during development may lead to latent and transgenerational adverse health effects. To understand the role of DNA methylation in these effects, we used zebrafish as a vertebrate model to investigate heritable changes in DNA methylation following chemical-induced stress during early development. We exposed zebrafish embryos to non-embryotoxic concentrations of the biologically active phthalate metabolite mono(2-ethylhexyl) phthalate (MEHP, 30 µM) and the DNA methyltransferase 1 inhibitor 5-azacytidine (5AC, 10 µM). Direct, latent and transgenerational effects on DNA methylation were assessed using global, genome-wide and locus-specific DNA methylation analyses.
resultsFollowing direct exposure in zebrafish embryos from 0 to 6 days post-fertilization, genome-wide analysis revealed a multitude of differentially methylated regions, strongly enriched at conserved non-genic elements for both compounds. Pathways involved in adipogenesis were enriched with the putative obesogenic compound MEHP. Exposure to 5AC resulted in enrichment of pathways involved in embryonic development and transgenerational effects on larval body length. Locus-specific methylation analysis of 10 differentially methylated sites revealed six of these loci differentially methylated in sperm sampled from adult zebrafish exposed during development to 5AC, and in first and second generation larvae. With MEHP, consistent changes were found at 2 specific loci in first and second generation larvae.
conclusionsOur results suggest a functional role for DNA methylation on cis-regulatory conserved elements following developmental exposure to compounds. Effects on these regions are potentially transferred to subsequent generations.
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