ArticleStem cell research & therapy2017
Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheets.
Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities.Biomaterials science · 2023Article
- Can Cryopreservation in Assisted Reproductive Technology (ART) Induce Epigenetic Changes to Gametes and Embryos?Journal of clinical medicine · 2023Review
- Cryopreservation of Cell Sheets for Regenerative Therapy: Application of Vitrified Hydrogel Membranes.Gels (Basel, Switzerland) · 2023Review
- Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine.Stem cell research & therapy · 2022Review
- Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2022Article
- Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model.BMC biotechnology · 2021Article
- The effect of aging on the biological and immunological characteristics of periodontal ligament stem cells.Stem cell research & therapy · 2020Article
- Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro.International journal of molecular medicine · 2019Article
- Cell sheets of human dental pulp stem cells for future application in bone replacement.Clinical oral investigations · 2019Article
- Tanshinone IIA promotes osteogenic differentiation of human periodontal ligament stem cells via ERK1/2-dependent Runx2 induction.American journal of translational research · 2019Article
- Cryopreserved clumps of mesenchymal stem cell/extracellular matrix complexes retain osteogenic capacity and induce bone regeneration.Stem cell research & therapy · 2018Article
- Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets.Journal of periodontal & implant science · 2017Article
- Evaluation of periodontal ligament stem cell viability and proliferation on native collagen membranes: AnJournal of Indian Society of PeriodontologyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryopreservation has been extensively applied to the long-term storage of a diverse range of biological materials. However, no comprehensive study is currently available on the cryopreservation of periodontal ligament stem cell (PDLSC) sheets which have been suggested as excellent transplant materials for periodontal tissue regeneration. The aim of this study is to investigate the effect of cryopreservation on the structural integrity and functional viability of PDLSC sheets.
methodsPDLSC sheets prepared from extracted human molars were divided into two groups: the cryopreservation group (cPDLSC sheets) and the freshly prepared control group (fPDLSC sheets). The cPDLSC sheets were cryopreserved in a solution consisting of 90% fetal bovine serum and 10% dimethyl sulfoxide for 3 months. Cell viability and cell proliferation rates of PDLSCs in both groups were evaluated by cell viability assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, respectively. The multilineage differentiation potentials of the cells were assessed by von Kossa staining and Oil Red O staining. The chromosomal stability was examined by karyotype analysis. Moreover, the cell sheets in each group were transplanted subcutaneously into the dorsal site of nude mice, after which Sirius Red staining was performed to analyze the efficiency of tissue regeneration.
resultsThe PDLSCs derived from both groups of cell sheets showed no significant difference in their viability, proliferative capacities, and multilineage differentiation potentials, as well as chromosomal stability. Furthermore, transplantation experiments based on a mouse model demonstrated that the cPDLSC sheets were equally effective in generating viable osteoid tissues in vivo as their freshly prepared counterparts. In both cases, the regenerated tissues showed similar network patterns of bone-like matrix.
conclusionsOur results offer convincing evidence that cryopreservation does not alter the biological properties of PDLSC sheets and could enhance their clinical utility in tissue regeneration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.