ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2017
Bud detachment in hydra requires activation of fibroblast growth factor receptor and a Rho-ROCK-myosin II signaling pathway to ensure formation of a basal constriction.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Hydra at the Crossroads of Evolution: Insights into Cell-type Diversification, Regeneration, and Epigenetic Regulation.Results and problems in cell differentiation · 2026Review
- Actin-binding protein tropomodulin-1 is necessary for basal constriction formation and bud detachment iniScience · 2025Article
- Signaling Mechanism of Budding, Proliferation, and Tissue Regeneration in Cnidaria.Current issues in molecular biology · 2025Review
- Environmental and molecular regulation of asexual reproduction in the sea anemoneRoyal Society open science · 2023Article
- Article
- New developments in the biology of fibroblast growth factors.WIREs mechanisms of disease · 2022Review
- Binding Pattern Reconstructions of FGF-FGFR Budding-Inducing Signaling in Reef-Building Corals.Frontiers in physiology · 2021Article
- Review
- Cell Sorting in Hydra vulgaris Arises from Differing Capacities for Epithelialization between Cell Types.Current biology : CB · 2020Article
- What lies beneath: Hydra provides cnidarian perspectives into the evolution of FGFR docking proteins.Development genes and evolution · 2020Article
- Curling of epithelial monolayers reveals coupling between active bending and tissue tension.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Basal epithelial tissue folding is mediated by differential regulation of microtubules.Development (Cambridge, England) · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHydra propagates asexually by exporting tissue into a bud, which detaches 4 days later as a fully differentiated young polyp. Prerequisite for detachment is activation of fibroblast growth factor receptor (FGFR) signaling. The mechanism which enables constriction and tissue separation within the monolayered ecto- and endodermal epithelia is unknown.
resultsHistological sections and staining of F-actin by phalloidin revealed conspicuous cell shape changes at the bud detachment site indicating a localized generation of mechanical forces and the potential enhancement of secretory functions in ectodermal cells. By gene expression analysis and pharmacological inhibition, we identified a candidate signaling pathway through Rho, ROCK, and myosin II, which controls bud base constriction and rearrangement of the actin cytoskeleton. Specific regional myosin phosphorylation suggests a crucial role of ectodermal cells at the detachment site. Inhibition of FGFR, Rho, ROCK, or myosin II kinase activity is permissive for budding, but represses myosin phosphorylation, rearrangement of F-actin and constriction. The young polyp remains permanently connected to the parent by a broad tissue bridge.
conclusionsOur data suggest an essential role of FGFR and a Rho-ROCK-myosin II pathway in the control of cell shape changes required for bud detachment. Developmental Dynamics 246:502-516, 2017. © 2017 The Authors Developmental Dynamics published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.
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