Evidence map›Paper›PMID 28409998›Full record

ArticleBioengineered2017

Suitability of various DNA extraction methods for a traditional Chinese paocai system.

Nan Zhao, Jialiang Cai, Chuchu Zhang, Zhuang Guo, Wenwei Lu, Bo Yang, Feng-Wei Tian, Xiao-Ming Liu, Hao Zhang, Wei Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. International journal of microbiology · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nan Zhaoa State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.
Jialiang Caia State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.
Chuchu Zhanga State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.
Zhuang Guod Northwest Hubei Research Institute of Traditional Fermented Food, College of Chemical Engineering and Food Science, Hu Bei University of Arts and Science , Xiangyang , China.
Wenwei Lua State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.
Bo Yanga State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.
Feng-Wei Tiana State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.
Xiao-Ming Liua State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.
Hao Zhanga State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.
Wei Chena State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traditional paocai brine (PB), which is continuously propagated by back-slopping and contains various species of lactic acid bacteria (LAB), is critical for the flavor of paocai. Culture-independent approaches are commonly used to investigate the microbial communities of fermented food. To evaluate the influence of different DNA (DNA) extraction methods on estimates of bacterial community profiles from 4 PBs, the lysis efficiency, DNA yield, purity and denaturing gradient gel electrophoresis (DGGE) profiles of V3 region of a 16S ribosomal ribonucleic acid gene were acquired. The cell lysis pattern of SDS + beads and Lysing matrix E+ beads (methods 3 and 4) showed higher cell lysis efficiency than SDS and SDS + Lysozyme (methods 1 and 2) in all PBs. SDS + beads obtained the largest DNA yield of the 4 methods. Moreover, methods 3 and 4 resulted in higher H' values and generated more global bacteria profiles than other methods. Overall, our results demonstrate that the properties of PB significantly affect the efficiency of DNA extraction methods. Methods 3 and 4 were both suitable for DNA extraction from PB. Method 3 is more economic, simple and rapid than method 4 for large-scale studies of the bacterial profiles of PB.

Indexed as

BacteriaBacterial Typing TechniquesChemical FractionationDNA, BacterialFood AnalysisFood MicrobiologySpecimen HandlingVegetablesDNA, Bacterial16S rDNAdiversityDNA extractionFermented vegetablesPaocai

Identifiers

PMID28409998
PMCPMC5639867

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