ArticleInnate immunity2017
High-affinity caspase-4 binding to LPS presented as high molecular mass aggregates or in outer membrane vesicles.
Article in Innate immunity, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- When folding CARDs wins the game: molecular snapshots of lipopolysaccharide sensing by the non-canonical inflammasome.Biochemical Society transactions · 2026Review
- ALDH2 Mediated Ferroptosis Regulation in Ischemia-Reperfusion Injury.Journal of cellular and molecular medicine · 2026Review
- Beyond the Toll-Like Receptor 4. Structure-Dependent Lipopolysaccharide Recognition Systems: How far are we?ChemMedChem · 2025Review
- Recognition ofJournal of oral microbiology · 2025Article
- NLRP11 is a pattern recognition receptor for bacterial lipopolysaccharide in the cytosol of human macrophages.Science immunology · 2023Article
- LPS-aggregating proteins GBP1 and GBP2 are each sufficient to enhance caspase-4 activation both in cellulo and in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Role of Caspase-11 and Pyroptosis in the Regulation of Inflammation in Peri-Implantitis.Journal of inflammation research · 2023Review
- SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade.The EMBO journal · 2021Article
- Human guanylate binding proteins: nanomachines orchestrating host defense.The FEBS journal · 2021Review
- An overview of the non-canonical inflammasome.Molecular aspects of medicine · 2020Review
- Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions.The EMBO journal · 2020Article
- Caspase-11-mediated enteric neuronal pyroptosis underlies Western diet-induced colonic dysmotility.The Journal of clinical investigation · 2020Article
- Lipopolysaccharide Recognition in the Crossroads of TLR4 and Caspase-4/11 Mediated Inflammatory Pathways.Frontiers in immunology · 2020Review
- Review
- Detecting lipopolysaccharide in the cytosol of mammalian cells: Lessons from MD-2/TLR4.Journal of leukocyte biology · 2019Review
- An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS).PLoS pathogens · 2019Article
- Innate immunity to intracellular LPS.Nature immunology · 2019Review
- Caspase-4 disaggregates lipopolysaccharide micelles via LPS-CARD interaction.Scientific reports · 2019Article
- Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish.Nature communications · 2018Article
- LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation.The EMBO journal · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Caspases of the non-canonical inflammasome (caspases -4, -5, and -11) directly bind endotoxin (LOS/LPS) and can be activated in the absence of any co-factors. Models of LPS-induced caspase activation have postulated that 1:1 binding of endotoxin monomers to caspase trigger caspase oligomerization and activation, analogous to that established for endotoxin-induced activation of MD-2/TLR4. However, using metabolically radiolabeled LOS and LPS, we now show high affinity and selective binding of caspase-4 to high molecular mass aggregates of purified endotoxin and to endotoxin-rich outer membrane vesicles without formation of 1:1 endotoxin:caspase complexes. Thus, our findings demonstrate markedly different endotoxin recognition properties of caspase-4 from that of MD-2/TLR4 and strongly suggest that activation of caspase-4 (and presumably caspase-5 and caspase-11) are mediated by interactions with activating endotoxin-rich membrane interfaces rather than by endotoxin monomers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.