Evidence map›Paper›PMID 28408783›Full record

ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2017

Prokaryotic expression of the extracellular domain of porcine programmed death 1 (PD-1) and its ligand PD-L1 and identification of the binding with peripheral blood mononuclear cells

Yan-Ping Zhu, Feng Yue, Yong He, Peng Li, Yuan Yang, Yu-Ting Han, Yan-Fang Zhang, Guo-Peng Sun, Dong-Guang Guo, Mei Yin and 1 more

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it, 2 citations in OpenAlex.

  1. Pooled it
  2. Frontiers in cellular and infection microbiology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Yan-Ping ZhuBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Feng YueBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Yong HeBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Peng LiBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Yuan YangBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Yu-Ting HanBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Yan-Fang ZhangBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Guo-Peng SunBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Dong-Guang GuoBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Mei YinBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Xuan-Nian WangBiotechnology Research Center, School of Life Science and Technology, Xinxiang University, Xinxiang, Henan Province 453003, China (Zhu, Yue, He, Li, Yang, Han, Zhang, Sun, Guo, Wang); College of Veterinary and Animal Sciences, Henan Institute of Science and Technology, Xinxiang, Henan Province 453003, China (Yang, Han, Yin, Wang).
Xinxiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Programmed cell death protein 1 (PD-1), a costimulatory molecule of the CD28 family, has 2 ligands, PD-L1 and PD-L2. Our previous studies showed that the expression of PD-1 and PD-L1 is up-regulated during viral infection in pigs. Extensive studies have shown that blockade of the PD-1/PD-L1 pathways by anti-PD-L1 antibody or soluble PD-1 restores exhausted T-cells in humans and mice. In the present study the extracellular domains of PD-1 and PD-L1 were used to evaluate the binding of PD-1 and PD-L1 with peripheral blood mononuclear cells (PBMCs). We amplified the cDNA encoding the extracellular domains of PD-1 and PD-L1 to construct recombinant expression plasmids and obtain soluble recombinant proteins, which were then labeled with fluorescein isothiocyanate (FITC). The His-

Indexed as

SwineAnimalsAntibodiesB7-H1 AntigenCell AdhesionCloning, MolecularFemaleLeukocytes, MononuclearProgrammed Cell Death 1 ReceptorProtein DomainsRabbitsAntibodiesB7-H1 AntigenProgrammed Cell Death 1 Receptor

Identifiers

PMID28408783
PMCPMC5370541
OpenAlexW2946828042

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.