ArticleGenome research2017
Extending the spectrum of DNA sequences retrieved from ancient bones and teeth.
Article in Genome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 118 citations in OpenAlex.
- Ancient DNA and Human Physiology.Physiology (Bethesda, Md.) · 2026Review
- Ancient wheat DNA: How and what for?Journal, genetic engineering & biotechnology · 2026Article
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- Investigating ancient human DNA preservation on cave walls and in rock art.Nature communications · 2026Article
- Review
- CarpeDeam: a de novo metagenome assembler for heavily damaged ancient datasets.Genome biology · 2025Article
- Optimizing ancient DNA recovery from archaeological plant seeds.Scientific reports · 2025Article
- A Post-Mortem Molecular Damage Profile in the Ancient Human Mitochondrial DNA.Molecular ecology resources · 2025Article
- Colonization history of snow algae on Hawai'i island.The ISME journal · 2025Article
- Improved detection of methylation in ancient DNA.Genome biology · 2024Article
- Maximizing efficiency in sedimentary ancient DNA analysis: a novel extract pooling approach.Scientific reports · 2024Article
- Review
- The origins and diversification of Holarctic brown bear populations inferred from genomes of past and present populations.Proceedings. Biological sciences · 2024Article
- Genome sequences of 36,000- to 37,000-year-old modern humans at Buran-Kaya III in Crimea.Nature ecology & evolution · 2023Article
- Review: Computational analysis of human skeletal remains in ancient DNA and forensic genetics.iScience · 2023Review
- Review
- A 14th century CE Brucella melitensis genome and the recent expansion of the Western Mediterranean clade.PLoS pathogens · 2023Article
- Article
- DNA and protein analyses of hair in forensic genetics.International journal of legal medicine · 2023Review
- Extended longevity of DNA preservation in Levantine Paleolithic sediments, Sefunim Cave, Israel.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The number of DNA fragments surviving in ancient bones and teeth is known to decrease with fragment length. Recent genetic analyses of Middle Pleistocene remains have shown that the recovery of extremely short fragments can prove critical for successful retrieval of sequence information from particularly degraded ancient biological material. Current sample preparation techniques, however, are not optimized to recover DNA sequences from fragments shorter than ∼35 base pairs (bp). Here, we show that much shorter DNA fragments are present in ancient skeletal remains but lost during DNA extraction. We present a refined silica-based DNA extraction method that not only enables efficient recovery of molecules as short as 25 bp but also doubles the yield of sequences from longer fragments due to improved recovery of molecules with single-strand breaks. Furthermore, we present strategies for monitoring inefficiencies in library preparation that may result from co-extraction of inhibitory substances during DNA extraction. The combination of DNA extraction and library preparation techniques described here substantially increases the yield of DNA sequences from ancient remains and provides access to a yet unexploited source of highly degraded DNA fragments. Our work may thus open the door for genetic analyses on even older material.
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