Evidence map›Paper›PMID 28407771›Full record

ArticleBMC veterinary research2017

Amyloid precursor protein, lipofuscin accumulation and expression of autophagy markers in aged bovine brain.

D De Biase, A Costagliola, T B Pagano, G Piegari, S Wojcik, J Dziewiątkowski, E Grieco, G Mattace Raso, V Russo, S Papparella and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 20 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Molecular and cellular pathways contributing to brain aging.Behavioral and brain functions : BBF · 2021
    Review
  9. Review
  10. Review
  11. Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

D De BiaseDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II via Delpino, 1, 80137, Naples, Italy.
A CostagliolaDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II via Delpino, 1, 80137, Naples, Italy. alessandro.costagliola@unina.it.
T B PaganoDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II via Delpino, 1, 80137, Naples, Italy.
G PiegariDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II via Delpino, 1, 80137, Naples, Italy.
S WojcikDepartment of Anatomy and Neurobiology, Medical University of Gdansk, Debinki 1 80-11, Gdansk, Poland.
J DziewiątkowskiDepartment of Anatomy and Neurobiology, Medical University of Gdansk, Debinki 1 80-11, Gdansk, Poland.
E GriecoAzienda Sanitaria Locale, Salerno, Italy.
G Mattace RasoDepartment of Pharmacy, University of Naples Federico II, Via Montesano 49, 80131, Naples, Italy.
V RussoDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II via Delpino, 1, 80137, Naples, Italy.
S PapparellaDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II via Delpino, 1, 80137, Naples, Italy.
O PacielloDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II via Delpino, 1, 80137, Naples, Italy.
University of Naples Federico II · ITGdańsk Medical University · PLASL Roma · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAutophagy is a highly regulated process involving the bulk degradation of cytoplasmic macromolecules and organelles in mammalian cells via the lysosomal system. Dysregulation of autophagy is implicated in the pathogenesis of many neurodegenerative diseases and integrity of the autophagosomal - lysosomal network appears to be critical in the progression of aging. Our aim was to survey the expression of autophagy markers and Amyloid precursor protein (APP) in aged bovine brains. For our study, we collected samples from the brain of old (aged 11-20 years) and young (aged 1-5 years) Podolic dairy cows. Formalin-fixed and paraffin embedded sections were stained with routine and special staining techniques. Primary antibodies for APP and autophagy markers such as Beclin-1 and LC3 were used to perform immunofluorescence and Western blot analysis.

resultsHistologically, the most consistent morphological finding was the age-related accumulation of intraneuronal lipofuscin. Furthermore, in aged bovine brains, immunofluorescence detected a strongly positive immunoreaction to APP and LC3. Beclin-1 immunoreaction was weak or absent. In young controls, the immunoreaction for Beclin-1 and LC3 was mild while the immunoreaction for APP was absent. Western blot analysis confirmed an increased APP expression and LC3-II/LC3-I ratio and a decreased expression of Beclin-1 in aged cows.

conclusionsThese data suggest that, in aged bovine, autophagy is significantly impaired if compared to young animals and they confirm that intraneuronal APP deposition increases with age.

Indexed as

AutophagyAgingAmyloid beta-Protein PrecursorAnimalsBeclin-1BiomarkersBlotting, WesternBrainCattleFemaleLipofuscinMembrane ProteinsAmyloid beta-Protein PrecursorBeclin-1BiomarkersLipofuscinMembrane ProteinsAgeingAutophagyBovineBrainNeuropathology

Identifiers

PMID28407771
PMCPMC5390414
OpenAlexW2606676059

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.