Evidence map›Paper›PMID 28407546›Full record

ArticleChemosphere2017

Exploring adduct formation between human serum albumin and eleven organophosphate ester flame retardants and plasticizers using MALDI-TOF/TOF and LC-Q/TOF.

Shaogang Chu, Margaret R Baker, Gladys Leong, Robert J Letcher, Shirley J Gee, Bruce D Hammock, Qing X Li

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemosphere, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.0field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Shaogang ChuDepartment of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East West Road, Honolulu, HI, 96822, USA; Ecotoxicology and Wildlife Health Division, Wildlife and Landscape Science Directorate, Science and Technology Branch, National Wildlife Research Centre, Environment and Climate Change Canada, 1125 Colonel Bay Dr., Ottawa, ON, K1A 0H3, Canada.
Margaret R BakerDepartment of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East West Road, Honolulu, HI, 96822, USA.
Gladys LeongDepartment of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East West Road, Honolulu, HI, 96822, USA.
Robert J LetcherEcotoxicology and Wildlife Health Division, Wildlife and Landscape Science Directorate, Science and Technology Branch, National Wildlife Research Centre, Environment and Climate Change Canada, 1125 Colonel Bay Dr., Ottawa, ON, K1A 0H3, Canada.
Shirley J GeeDepartment of Entomology and Nematology, University of California at Davis, One Shields Avenue, Davis, CA, 95616-858, USA.
Bruce D HammockDepartment of Entomology and Nematology, University of California at Davis, One Shields Avenue, Davis, CA, 95616-858, USA.
Qing X LiDepartment of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East West Road, Honolulu, HI, 96822, USA. Electronic address: qingl@hawaii.edu.
University of Hawaiʻi at Mānoa · USUniversity of California, Davis · USEnvironment and Climate Change Canada · CA

Funding

Western Center for Agricultural Health and SafetyU54OH007550 · OH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI FATHALLAH, FADI A · 2011 to 2025
$27.5M
RESEARCH DESIGN, BIOSTATISTICS AND RESEARCH ETHICS COREG12MD007601 · NIMHD · UNIVERSITY OF HAWAII AT MANOA · PI BERRY, MARLA J · 2012 to 2016
$13.0M
HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINSR01ES002710 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI HAMMOCK, BRUCE D · 1985 to 2018
$4.0M
ACL HHS U54OH007550NIEHS NIH HHS R01 ES002710NIMHD NIH HHS G12 MD007601NIOSH CDC HHS U54 OH007550
6 · The paper itself

Abstract

Organophosphate (OP) and organophosphate ester (OPE) adducts of albumin are valuable biomarkers for retrospective verification of exposure. In the present study, our goal was to determine whether OPE flame retardants (OPE FRs) and OPE plasticizers can covalently bind to human serum albumin (HSA), which would allow the resulting adducts to be used to evaluate exposure. Eleven OPE FRs and plasticizers were examined in a HSA-adduct in vitro assay. Pure HSA was incubated with the target OPEs, as well as with an OP insecticide (profenofos) positive control. After enzymatic cleavage with pepsin or Glu-C, the digested albumin was analyzed by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) and liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-Q-ToF-MS). Under optimized HSA assay conditions, tyrosine adducts were formed at Y

Indexed as

BiomarkersChromatography, LiquidDNA AdductsFlame RetardantsHumansOrganophosphatesOrganophosphorus CompoundsPhosphorylationPlasticizersRetrospective StudiesSerum AlbuminSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTyrosineBiomarkersDNA AdductsFlame RetardantsOrganophosphatesOrganophosphorus CompoundsPlasticizersSerum AlbuminTyrosineBiomarkerFlame retardantHuman serum albuminOrganophosphate esterPlasticizerProtein adduct

Identifiers

PMID28407546
PMCPMC5494263
OpenAlexW2598270026

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.