ArticleThe New phytologist2018
Phytaspase-mediated precursor processing and maturation of the wound hormone systemin.
Article in The New phytologist, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- The front line of defence: a meta-analysis of apoplastic proteases in plant immunity.Journal of experimental botany · 2021Pooled it
- Mycorrhiza-induced resistance against Botrytis cinerea in tomato operates through the systemin signalling cascade.The New phytologist · 2026Article
- Processing and release of the maize phytocytokine Zip1.Plant physiology · 2026Article
- Plant Peptide Hormones: Distinctive Horizons in Plant Development and Stress Resilience.Plant, cell & environment · 2026Review
- Processing and release of endogenous immunogenic peptide signals.The Plant journal : for cell and molecular biology · 2026Review
- Plant subtilases as key initiators of peptide ligand-receptor signaling.The Plant journal : for cell and molecular biology · 2026Review
- Plant caspase-like proteins: from function identification to application in winter rapeseed genetic breeding.Frontiers in plant science · 2026Review
- A fungal pathogen manipulates phytocytokine signaling for plant infection.Nature communications · 2025Article
- Long-Term Phytaspase Responses inInternational journal of molecular sciences · 2025Article
- Poltergeist-Like 2 (PLL2)-dependent activation of herbivore defence distinguishes systemin from other immune signalling pathways.Nature plants · 2025Article
- Retrograde Transport of Tobacco Phytaspase Is Mediated by Its Partner, Tubby-like F-Box Protein 8.International journal of molecular sciences · 2025Article
- Peptide hormones in plants.Molecular horticulture · 2025Review
- The lowdown on breakdown: Open questions in plant proteolysis.The Plant cell · 2024Review
- Proteolysis in plant immunity.The Plant cell · 2024Review
- Pull the fuzes: Processing protein precursors to generate apoplastic danger signals for triggering plant immunity.Plant communications · 2024Review
- Characterization of Phytaspase Proteolytic Activity Using Fluorogenic Peptide Substrates.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Purification of Phytaspases Using a Biotinylated Peptide Inhibitor.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Phytaspase Is Capable of Detaching the Endoplasmic Reticulum Retrieval Signal from Tobacco Calreticulin-3.International journal of molecular sciences · 2023Article
- A sensitive and accurate method for quantifying endogenous systemin levels and verifying natural occurrence of Leu-Systemin.Plant communications · 2023Article
- Small Signals Lead to Big Changes: The Potential of Peptide-Induced Resistance in Plants.Journal of fungi (Basel, Switzerland) · 2023Review
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Authors and funding
13 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peptide hormones are implicated in many important aspects of plant life and are usually synthesized as precursor proteins. In contrast to animals, data for plant peptide hormone maturation are scarce and the specificity of processing enzyme(s) is largely unknown. Here we tested a hypothesis that processing of prosystemin, a precursor of tomato (Solanum lycopersicum) wound hormone systemin, is performed by phytaspases, aspartate-specific proteases of the subtilase family. Following the purification of phytaspase from tomato leaves, two tomato phytaspase genes were identified, the cDNAs were cloned and the recombinant enzymes were obtained after transient expression in Nicotiana benthamiana. The newly identified tomato phytaspases hydrolyzed prosystemin at two aspartate residues flanking the systemin sequence. Site-directed mutagenesis of the phytaspase cleavage sites in prosystemin abrogated not only the phytaspase-mediated processing of the prohormone in vitro, but also the ability of prosystemin to trigger the systemic wound response in vivo. The data show that the prohormone prosystemin requires processing for signal biogenesis and biological activity. The identification of phytaspases as the proteases involved in prosystemin maturation provides insight into the mechanisms of wound signaling in tomato. Our data also suggest a novel role for cell death-related proteases in mediating defense signaling in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.