ArticleNucleic acids research2017
A network of cis and trans interactions is required for ParB spreading.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- A dual-function regulatory element couples ParB expression and DNA substrate specificity.bioRxiv : the preprint server for biology · 2026Article
- Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding.Nucleic acids research · 2026Article
- Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Single-molecule DNA-flow stretching assay as a versatile hybrid tool for investigating DNA-protein interactions.BMB reports · 2025Review
- Phase-separated ParB enforces diverse DNA compaction modes and stabilizes the parS-centered partition complex.Nucleic acids research · 2024Article
- Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells.Nature communications · 2024Article
- Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.FEMS microbiology reviews · 2024Review
- Dynamic ParB-DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation.Nucleic acids research · 2023Article
- A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments.Cell reports methods · 2023Article
- Partition complex structure can arise from sliding and bridging of ParB dimers.Nature communications · 2023Article
- ParB proteins can bypass DNA-bound roadblocks via dimer-dimer recruitment.Science advances · 2022Article
- CTP andeLife · 2021Article
- CTP promotes efficient ParB-dependent DNA condensation by facilitating one-dimensional diffusion fromeLife · 2021Article
- Compaction and control-the role of chromosome-organizing proteins in Streptomyces.FEMS microbiology reviews · 2020Review
- Bacterial chromosome segregation by the ParABS system.Open biology · 2020Review
- Article
- Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes.Microorganisms · 2020Review
- Chromosome Segregation Proteins as Coordinators of Cell Cycle in Response to Environmental Conditions.Frontiers in microbiology · 2020Review
- Addressing the role of centromere sites in activation of ParB proteins for partition complex assembly.PloS one · 2020Article
- Potentiation of curing by a broad-host-range self-transmissible vector for displacing resistance plasmids to tackle AMR.PloS one · 2020Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most bacteria utilize the highly conserved parABS partitioning system in plasmid and chromosome segregation. This system depends on a DNA-binding protein ParB, which binds specifically to the centromere DNA sequence parS and to adjacent non-specific DNA over multiple kilobases in a phenomenon called spreading. Previous single-molecule experiments in combination with genetic, biochemical and computational studies have argued that ParB spreading requires cooperative interactions between ParB dimers including DNA bridging and possible nearest-neighbor interactions. A recent structure of a ParB homolog co-crystallized with parS revealed that ParB dimers tetramerize to form a higher order nucleoprotein complex. Using this structure as a guide, we systematically ablated a series of proposed intermolecular interactions in the Bacillus subtilis ParB (BsSpo0J) and characterized their effect on spreading using both in vivo and in vitro assays. In particular, we measured DNA compaction mediated by BsSpo0J using a recently developed single-molecule method to simultaneously visualize protein binding on single DNA molecules and changes in DNA conformation without protein labeling. Our results indicate that residues acting as hubs for multiple interactions frequently led to the most severe spreading defects when mutated, and that a network of both cis and trans interactions between ParB dimers is necessary for spreading.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.