ArticlePloS one2017
Functional and structural characterization of plastidic starch phosphorylase during barley endosperm development.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Starch phosphorylase acts as a pivotal scaffold connecting starch metabolism enzymes with photosystem I subunits.Plant & cell physiology · 2026Article
- HvPHO1 Controls a Glucose 1-Phosphate-SnRK1 Signalling Module That Coordinates Starch Accumulation in Barley Endosperm.Plant biotechnology journal · 2026Article
- A four-dimensional spatial transcriptome atlas of barley caryopsis development and germination.The Plant cell · 2026Article
- The plastidial PHS1-DPE1 complex drives efficient malto-oligosaccharides synthesis in rice starch metabolism.Nature communications · 2026Article
- Unveiling the genetic determinants of germination efficiency in common bermudagrass: A genome-wide association study.The plant genome · 2026Article
- A structural perspective on α-glucan catabolism in oxygenic phototrophs.The Plant journal : for cell and molecular biology · 2026Review
- Biochemical characterization and preliminary X-ray crystallographic analysis of cyanobacterial α-glucan phosphorylases.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Old player, new roles: defining the role of the plastidial phosphorylase.The New phytologist · 2025Review
- Impact of Temperature Stresses on Wheat Quality: A Focus on Starch and Protein Composition.Foods (Basel, Switzerland) · 2025Review
- Nuclear factor-Y-polycomb repressive complex2 dynamically orchestrates starch and seed storage protein biosynthesis in wheat.The Plant cell · 2024Article
- Kinetic and Structural Studies of the PlastidialACS omega · 2024Article
- Site-Directed Mutations at Phosphorylation Sites inPlants (Basel, Switzerland) · 2023Article
- Low-temperature at booting reduces starch content and yield of wheat by affecting dry matter transportation and starch synthesis.Frontiers in plant science · 2023Article
- Article
- Comparative Study of Starch Phosphorylase Genes and Encoded Proteins in Various Monocots and Dicots with Emphasis on Maize.International journal of molecular sciences · 2022Article
- Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases.International journal of molecular sciences · 2022Review
- E, K, B5, B6, and B9 vitamins and their specific immunological effects evaluated by flow cytometry.Frontiers in medicine · 2022Review
- Molecular Functions and Pathways of Plastidial Starch Phosphorylase (PHO1) in Starch Metabolism: Current and Future Perspectives.International journal of molecular sciences · 2021Review
- Starch biosynthesis in cereal endosperms: An updated review over the last decade.Plant communications · 2021Review
- Genome-Wide Identification and Characterization of the Brassinazole-resistant (International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of starch is essential for human nutrition and represents a major metabolic flux in the biosphere. The biosynthesis of starch in storage organs like barley endosperm operates via two main pathways using different substrates: starch synthases use ADP-glucose to produce amylose and amylopectin, the two major components of starch, whereas starch phosphorylase (Pho1) uses glucose-1-phosphate (G1P), a precursor for ADP-glucose production, to produce α-1,4 glucans. The significance of the Pho1 pathway in starch biosynthesis has remained unclear. To elucidate the importance of barley Pho1 (HvPho1) for starch biosynthesis in barley endosperm, we analyzed HvPho1 protein production and enzyme activity levels throughout barley endosperm development and characterized structure-function relationships of HvPho1. The molecular mechanisms underlying the initiation of starch granule biosynthesis, that is, the enzymes and substrates involved in the initial transition from simple sugars to polysaccharides, remain unclear. We found that HvPho1 is present as an active protein at the onset of barley endosperm development. Notably, purified recombinant protein can catalyze the de novo production of α-1,4-glucans using HvPho1 from G1P as the sole substrate. The structural properties of HvPho1 provide insights into the low affinity of HvPho1 for large polysaccharides like starch or amylopectin. Our results suggest that HvPho1 may play a role during the initiation of starch biosynthesis in barley.
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