ArticlePloS one2017
Dual effect of fetal bovine serum on early development depends on stage-specific reactive oxygen species demands in pigs.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- Modulation ofInternational journal of molecular sciences · 2026Article
- Effect of Vitrification on Lipidomics in Porcine Cumulus-Oocyte Complexes After In Vitro Maturation.Cells · 2026Article
- Comparative Study of Redox Status of MDCK Cells in Chicken Embryo Extract Versus Fetal Bovine Serum.International journal of molecular sciences · 2026Article
- Lycopene enhances epigenetic reprogramming and zygotic genome activation in the porcine somatic cell nuclear transfer embryo.Scientific reports · 2025Article
- Fetal Bovine Serum Supplementation Enhances Functional Consistency of IGRA Results in Bovine Tuberculosis Diagnostics.Animals : an open access journal from MDPI · 2025Article
- Comparative Proteomic Characterization of Serum-Free Cultivated HepG2 Cells Reveals Upregulated Drug Metabolism and Increased Oxidative Stress Protection.Journal of proteome research · 2025Article
- Improving porcine in vitro blastocyst development using fetal bovine serum, amino acids, and insulin-transferrin-selenium.The Journal of reproduction and development · 2025Article
- The role of the pentose phosphate pathway on reproductive functions.Animal reproduction · 2025Review
- Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm.Animals : an open access journal from MDPI · 2023Article
- Beneficial Effects of Catalpol Supplementation during In Vitro Maturation of Porcine Cumulus-Oocyte Complexes.Antioxidants (Basel, Switzerland) · 2023Article
- Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway.Journal of animal science and biotechnology · 2023Article
- Targeted isolation of photoactive pigments from mushrooms yielded a highly potent new photosensitizer: 7,7'-biphyscion.Scientific reports · 2022Article
- ROMO1 is required for mitochondrial metabolism during preimplantation embryo development in pigs.Cell division · 2021Article
- Supplementation of fetal bovine serum increased the quality ofJournal of advanced veterinary and animal research · 2021Article
- Reactive oxygen and nitrogen species regulate porcine embryo development during pre-implantation period: A mini-review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- L-Proline Activates Mammalian Target of Rapamycin Complex 1 and Modulates Redox Environment in Porcine Trophectoderm Cells.Biomolecules · 2021Article
- Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis.Antioxidants (Basel, Switzerland) · 2021Article
- A comparison of the effects of fetal bovine serum and newborn calf serum on cell growth and maintenance of cryopreserved mouse spermatogonial stem cells.Molecular biology reports · 2020Article
- Article
- Feline embryo development in commercially available human media supplemented with fetal bovine serum.The Journal of veterinary medical science · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the application of numerous supplements to improve in vitro culture (IVC) conditions of mammalian cells, studies regarding the effect of fetal bovine serum (FBS) on mammalian early embryogenesis, particularly in relation to redox homeostasis, are lacking. Herein, we demonstrated that early development of in vitro-produced (IVP) porcine embryos highly depends on the combination of FBS supplementation timing and embryonic reactive oxygen species (ROS) requirements. Interestingly, FBS significantly reduced intracellular ROS levels in parthenogenetically activated (PA) embryos regardless of the developmental stage. However, the beneficial effect of FBS on early embryogenesis was found only during the late phase (IVC 4-6 days) treatment group. In particular, developmental competence parameters, such as blastocyst formation rate, cellular survival, total cell number and trophectoderm proportion, were markedly increased by FBS supplementation during the late IVC phase. In addition, treatment with FBS elevated antioxidant transcript levels during the late IVC phase. In contrast, supplementation with FBS during the entire period (1-6 days) or during the early IVC phase (1-2 days) greatly impaired the developmental parameters. Consistent with the results from PA embryos, the developmental competence of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) embryos were markedly improved by treatment with FBS during the late IVC phase. Moreover, the embryonic stage-specific effects of FBS were reversed by the addition of an oxidant and were mimicked by treatment with an antioxidant. These findings may increase our understanding of redox-dependent early embryogenesis and contribute to the large-scale production of high-quality IVP embryos.
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