ArticlePLoS biology2017
Water diffusion closely reveals neural activity status in rat brain loci affected by anesthesia.
Article in PLoS biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 89 citations in OpenAlex.
- Thalamus and consciousness: a systematic review on thalamic nuclei associated with consciousness.Frontiers in neurology · 2025Pooled it
- 40 Years of diffusion MRI in the brain: From history to emerging frontiers.Imaging neuroscience (Cambridge, Mass.) · 2026Review
- Considerations and recommendations from the ISMRM diffusion study group for preclinical diffusion MRI: Part 1: In vivo small-animal imaging.Magnetic resonance in medicine · 2025Review
- Mapping grey and white matter activity in the human brain with isotropic ADC-fMRI.Nature communications · 2025Article
- Diffusion MRI in 2-Month-Old Mouse Brain Predicts Alzheimer's Pathology Genotype.NMR in biomedicine · 2025Article
- Apparent Diffusion Coefficient fMRI shines light on white matter resting-state connectivity compared to BOLD.Communications biology · 2025Article
- Somatosensory-evoked response induces extensive diffusivity and kurtosis changes associated with neural activity in rodents.Imaging neuroscience (Cambridge, Mass.) · 2025Article
- Evaluating the dependence of ADC-fMRI on haemodynamics in breath-hold and resting-state conditions.Imaging neuroscience (Cambridge, Mass.) · 2025Article
- From Brownian motion to virtual biopsy: a historical perspective from 40 years of diffusion MRI.Japanese journal of radiology · 2024Review
- Relationship between regional volume changes and water diffusion in fixed marmoset brains: an in vivo and ex vivo comparison.Scientific reports · 2024Article
- Alx3 deficiency disrupts energy homeostasis, alters body composition, and impairs hypothalamic regulation of food intake.Cellular and molecular life sciences : CMLS · 2024Article
- Optogenetic activation of dorsal raphe serotonin neurons induces brain-wide activation.Nature communications · 2024Article
- Estimating the volume of penumbra in rodents using DTI and stack-based ensemble machine learning framework.European radiology experimental · 2024Article
- Local orchestration of distributed functional patterns supporting loss and restoration of consciousness in the primate brain.Nature communications · 2024Article
- High-Throughput Microelectrode Arrays for Precise Functional Localization of the Globus Pallidus Internus.Cyborg and bionic systems (Washington, D.C.) · 2024Article
- Association study of brain structure-function coupling and glymphatic system function in patients with mild cognitive impairment due to Alzheimer's disease.Frontiers in neuroscience · 2024Article
- From Black Holes Entropy to Consciousness: The Dimensions of the Brain Connectome.Entropy (Basel, Switzerland) · 2023Article
- Disentangling the impact of cerebrospinal fluid formation and neuronal activity on solute clearance from the brain.Fluids and barriers of the CNS · 2023Article
- Magnetic resonance imaging to assess the brain response to fasting in glioblastoma-bearing rats as a model of cancer anorexia.Cancer imaging : the official publication of the International Cancer Imaging Society · 2023Article
- Regional ADC values of the morphologically normal canine brain.Frontiers in veterinary science · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diffusion functional MRI (DfMRI) reveals neuronal activation even when neurovascular coupling is abolished, contrary to blood oxygenation level-dependent (BOLD) functional MRI (fMRI). Here, we show that the water apparent diffusion coefficient (ADC) derived from DfMRI increased in specific rat brain regions under anesthetic conditions, reflecting the decreased neuronal activity observed with local field potentials (LFPs), especially in regions involved in wakefulness. In contrast, BOLD signals showed nonspecific changes, reflecting systemic effects of the anesthesia on overall brain hemodynamics status. Electrical stimulation of the central medial thalamus nucleus (CM) exhibiting this anesthesia-induced ADC increase led the animals to transiently wake up. Infusion in the CM of furosemide, a specific neuronal swelling blocker, led the ADC to increase further locally, although LFP activity remained unchanged, and increased the current threshold awakening the animals under CM electrical stimulation. Oppositely, induction of cell swelling in the CM through infusion of a hypotonic solution (-80 milliosmole [mOsm] artificial cerebrospinal fluid [aCSF]) led to a local ADC decrease and a lower current threshold to wake up the animals. Strikingly, the local ADC changes produced by blocking or enhancing cell swelling in the CM were also mirrored remotely in areas functionally connected to the CM, such as the cingulate and somatosensory cortex. Together, those results strongly suggest that neuronal swelling is a significant mechanism underlying DfMRI.
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