ArticleGenes2017
Identification of Drought-Responsive MicroRNAs from Roots and Leaves of Alfalfa by High-Throughput Sequencing.
Article in Genes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- Tea Plant miR5368-p5 Negatively Regulates Resistance Against Lasiodiplodia theobromae Through Targeting the CsWRKY57-CsLRR-RLK Module.Molecular plant pathology · 2025Article
- Transcriptome and small RNA analysis reveal potential miRNA-mediated interactions involved in somatic embryogenesis of Taxodium hybrid 'zhongshanshan'.Plant cell reports · 2025Article
- Article
- Regulation of endogenous hormone and miRNA in leaves of alfalfa (Medicago sativa L.) seedlings under drought stress by endogenous nitric oxide.BMC genomics · 2024Article
- Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress.Plants (Basel, Switzerland) · 2023Article
- Changes in epigenetic features in legumes under abiotic stresses.The plant genome · 2023Review
- The miRNA-mRNA Regulatory Modules ofInternational journal of molecular sciences · 2023Article
- Integrated Analysis of miRNAome and Transcriptome Identify Regulators of Elm Seed Aging.Plants (Basel, Switzerland) · 2023Article
- Whole-Transcriptome Sequencing Reveals a ceRNA Regulatory Network Associated with the Process of Periodic Albinism under Low Temperature in Baiye No. 1 (International journal of molecular sciences · 2023Article
- Integrative Analysis of miRNAs and Their Targets Involved in Ray Floret Growth inInternational journal of molecular sciences · 2022Article
- Comparative analysis of herbivory responsive miRNAs to delineate pod borer (Helicoverpa armigera) resistance mechanisms in Cajanus cajan and its wild relative Cajanus scarabaeoides.Plant cell reports · 2022Article
- Objective Phenotyping of Root System Architecture Using Image Augmentation and Machine Learning in Alfalfa (Medicago sativa L.).Plant phenomics (Washington, D.C.) · 2022Article
- Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis.BMC plant biology · 2021Article
- Article
- MicroRNAs: Potential Targets for Developing Stress-Tolerant Crops.Life (Basel, Switzerland) · 2021Review
- Utilization of Transcriptome, Small RNA, and Degradome Sequencing to Provide Insights Into Drought Stress and Rewatering Treatment inFrontiers in plant science · 2021Article
- miRNA-mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte,Plants (Basel, Switzerland) · 2020Article
- Article
- Regulation of terpenoid biosynthesis by miRNA in Persicaria minor induced by Fusarium oxysporum.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alfalfa, an important forage legume, is an ideal crop for sustainable agriculture and a potential crop for bioenergy resources. Drought, one of the most common environmental stresses, substantially affects plant growth, development, and productivity. MicroRNAs (miRNAs) are newly discovered gene expression regulators that have been linked to several plant stress responses. To elucidate the role of miRNAs in drought stress regulation of alfalfa, a high-throughput sequencing approach was used to analyze 12 small RNA libraries comprising of four samples, each with three biological replicates. From the 12 libraries, we identified 348 known miRNAs belonging to 80 miRNA families, and 281 novel miRNAs, using Mireap software. Eighteen known miRNAs in roots and 12 known miRNAs in leaves were screened as drought-responsive miRNAs. With the exception of miR319d and miR157a which were upregulated under drought stress, the expression pattern of drought-responsive miRNAs was different between roots and leaves in alfalfa. This is the first study that has identified miR3512, miR3630, miR5213, miR5294, miR5368 and miR6173 as drought-responsive miRNAs. Target transcripts of drought-responsive miRNAs were computationally predicted. All 447 target genes for the known miRNAs were predicted using an online tool. This study provides a significant insight on understanding drought-responsive mechanisms of alfalfa.
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