Evidence map›Paper›PMID 28406185›Full record

ArticleScientific reports2017

Knockdown of stem cell regulator Oct4A in ovarian cancer reveals cellular reprogramming associated with key regulators of cytoskeleton-extracellular matrix remodelling.

Chantel Samardzija, David W Greening, Ruth Escalona, Maoshan Chen, Maree Bilandzic, Rodney Luwor, George Kannourakis, Jock K Findlay, Nuzhat Ahmed

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.6field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
  2. Clinical implications of epithelial-to-mesenchymal transition in cancers which potentially spread to peritoneum.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2025
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  17. Expression of LGR5 in oral squamous cell carcinoma and its correlation to vasculogenic mimicry.International journal of clinical and experimental pathology · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Chantel SamardzijaDepartment of Obstetrics and Gynaecology, University of Melbourne, Victoria 3052, Australia.
David W GreeningDepartment of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria 3086, Australia.
Ruth EscalonaDepartment of Obstetrics and Gynaecology, University of Melbourne, Victoria 3052, Australia.
Maoshan ChenDepartment of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria 3086, Australia.
Maree BilandzicThe Hudson Institute of Medical Research, Victoria 3168, Australia.
Rodney LuworDepartment of Surgery, University of Melbourne, Royal Melbourne Hospital, Victoria 3052, Australia.
George KannourakisFiona Elsey Cancer Research Institute, Ballarat, Victoria 3353, Australia.
Jock K FindlayDepartment of Obstetrics and Gynaecology, University of Melbourne, Victoria 3052, Australia.
Nuzhat AhmedDepartment of Obstetrics and Gynaecology, University of Melbourne, Victoria 3052, Australia.
University of Melbourne · AUFederation University · AULa Trobe University · AUHudson Institute of Medical Research · AUThe Royal Melbourne Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oct4A is a master regulator of self-renewal and pluripotency in embryonic stem cells. It is a well-established marker for cancer stem cell (CSC) in malignancies. Recently, using a loss of function studies, we have demonstrated key roles for Oct4A in tumor cell survival, metastasis and chemoresistance in in vitro and in vivo models of ovarian cancer. In an effort to understand the regulatory role of Oct4A in tumor biology, we employed the use of an ovarian cancer shRNA Oct4A knockdown cell line (HEY Oct4A KD) and a global mass spectrometry (MS)-based proteomic analysis to investigate novel biological targets of Oct4A in HEY samples (cell lysates, secretomes and mouse tumor xenografts). Based on significant differential expression, pathway and protein network analyses, and comprehensive literature search we identified key proteins involved with biologically relevant functions of Oct4A in tumor biology. Across all preparations of HEY Oct4A KD samples significant alterations in protein networks associated with cytoskeleton, extracellular matrix (ECM), proliferation, adhesion, metabolism, epithelial-mesenchymal transition (EMT), cancer stem cells (CSCs) and drug resistance was observed. This comprehensive proteomics study for the first time presents the Oct4A associated proteome and expands our understanding on the biological role of this stem cell regulator in carcinomas.

Indexed as

AnimalsBiomarkersCell Line, TumorCellular ReprogrammingComputational BiologyCytoskeletonDisease Models, AnimalExtracellular MatrixFemaleGene Knockdown TechniquesHumansImmunohistochemistryMiceNeoplastic Stem CellsOctamer Transcription Factor-3Ovarian NeoplasmsBiomarkersOctamer Transcription Factor-3POU5F1 protein, humanProteome

Identifiers

PMID28406185
PMCPMC5390261
OpenAlexW2606952191

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.