ArticlePhysical biology2017
The role of cell body density in ruminant retina mechanics assessed by atomic force and Brillouin microscopy.
Article in Physical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Biomechanics of the retina: experimental and clinical perspectives.Biomedical engineering online · 2026Review
- Highly dynamic mechanical transitions in embryonic cell populations during Drosophila gastrulation.Nature communications · 2025Article
- Article
- Optical coherence tomography-guided Brillouin microscopy highlights regional tissue stiffness differences during anterior neural tube closure in the Mthfd1l murine mutant.Development (Cambridge, England) · 2024Article
- Progressive alteration of murine bladder elasticity in actinic cystitis detected by Brillouin microscopy.Scientific reports · 2024Article
- Brillouin microscopy.Nature reviews. Methods primers · 2024Article
- Increasing cell culture density during a developmental window prevents fated rod precursors derailment toward hybrid rod-glia cells.Scientific reports · 2023Article
- Multimodal imaging system combining optical coherence tomography and Brillouin microscopy for neural tube imaging.Optics letters · 2022Article
- Retina organoids: Window into the biophysics of neuronal systems.Biophysics reviews · 2022Review
- Label-free histological imaging of tissues using Brillouin light scattering contrast.Biomedical optics express · 2021Article
- Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes.Nature protocols · 2021Article
- Characterization of retinal biomechanical properties using Brillouin microscopy.Journal of biomedical optics · 2020Article
- Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension.International journal of molecular sciences · 2020Article
- Microglia control vascular architecture via a TGFβ1 dependent paracrine mechanism linked to tissue mechanics.Nature communications · 2020Article
- Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development.Scientific reports · 2019Article
- Morpho-Rheological Fingerprinting of Rod Photoreceptors Using Real-Time Deformability Cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Mechanical changes of peripheral nerve tissue microenvironment and their structural basis during development.APL bioengineering · 2019Article
- Tissue mechanics, an important regulator of development and disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Tissue biomechanics during cranial neural tube closure measured by Brillouin microscopy and optical coherence tomography.Birth defects research · 2019Article
- Single etalon design for two-stage cross-axis VIPA spectroscopy.Biomedical optics express · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Cells in the central nervous system (CNS) respond to the stiffness of their environment. CNS tissue is mechanically highly heterogeneous, thus providing motile cells with region-specific mechanical signals. While CNS mechanics has been measured with a variety of techniques, reported values of tissue stiffness vary greatly, and the morphological structures underlying spatial changes in tissue stiffness remain poorly understood. We here exploited two complementary techniques, contact-based atomic force microscopy and contact-free Brillouin microscopy, to determine the mechanical properties of ruminant retinae, which are built up by different tissue layers. As in all vertebrate retinae, layers of high cell body densities ('nuclear layers') alternate with layers of low cell body densities ('plexiform layers'). Different tissue layers varied significantly in their mechanical properties, with the photoreceptor layer being the stiffest region of the retina, and the inner plexiform layer belonging to the softest regions. As both techniques yielded similar results, our measurements allowed us to calibrate the Brillouin microscopy measurements and convert the Brillouin shift into a quantitative assessment of elastic tissue stiffness with optical resolution. Similar as in the mouse spinal cord and the developing Xenopus brain, we found a strong correlation between nuclear densities and tissue stiffness. Hence, the cellular composition of retinae appears to strongly contribute to local tissue stiffness, and Brillouin microscopy shows a great potential for the application in vivo to measure the mechanical properties of transparent tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.