Evidence map›Paper›PMID 28405918›Full record

ArticleInternational journal of hematology2017

Hepcidin suppression in β-thalassemia is associated with the down-regulation of atonal homolog 8.

Supranee Upanan, Andrew T McKie, Gladys O Latunde-Dada, Sittiruk Roytrakul, Chairat Uthaipibull, Peraphan Pothacharoen, Prachya Kongtawelert, Suthat Fucharoen, Somdet Srichairatanakool

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Article in International journal of hematology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Supranee UpananDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Andrew T McKieDivision of Diabetes and Nutritional Sciences, School of Medicine, King's College London, London, SE1 9NH, UK.
Gladys O Latunde-DadaDivision of Diabetes and Nutritional Sciences, School of Medicine, King's College London, London, SE1 9NH, UK.
Sittiruk RoytrakulNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani, 12120, Thailand.
Chairat UthaipibullNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani, 12120, Thailand.
Peraphan PothacharoenDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Prachya KongtawelertDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Suthat FucharoenThalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakornpathom, 73170, Thailand.
Somdet SrichairatanakoolDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. ssrichai@med.cmu.ac.th.
Chiang Mai University · THKing's College London · GBMahidol University · THNational Center for Genetic Engineering and Biotechnology · THNational Science and Technology Development Agency · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Atonal homolog 8 (ATOH8) is defined as a positive regulator of hepcidin transcription, which links erythropoietic activity with iron-sensing molecules. In the present study, we investigated the association between hepcidin and ATOH8 expression in β-thalassemia. We found that inhibition of hepcidin expression in β-thalassemia is correlated with reduced ATOH8 expression. Hepatic hepcidin 1 (Hamp1) and Atoh8 mRNA expression were down-regulated in β-thalassemic mice. Hepcidin (HAMP) and ATOH8 mRNA expression were consistently suppressed in Huh7 cells cultured in medium supplemented with β-thalassemia patient serum. The Huh7 cells, which were transfected with ATOH8-FLAG expression plasmid and cultured in the supplemented medium, exhibited increased levels of ATOH8 mRNA, ATOH8-FLAG protein, pSMAD1,5,8, and HAMP mRNA. Interestingly, over-expression of ATOH8 reversed the effects of hepcidin suppression induced by the β-thalassemia patient sera. In conclusion, hepcidin suppression in β-thalassemia is associated with the down-regulation of ATOH8 in response to anemia. We, therefore, suggest that ATOH8 is an important transcriptional regulator of hepcidin in β-thalassemia.

Indexed as

Genetic Association StudiesAnimalsBasic Helix-Loop-Helix Proteinsbeta-ThalassemiaCells, CulturedDown-RegulationGene ExpressionHepcidinsHumansMice, Inbred C57BLMice, KnockoutAtoh8 protein, mouseBasic Helix-Loop-Helix ProteinsHepcidinsAnemiaATOH8HepcidinIron overloadβ-Thalassemia

Identifiers

PMID28405918
OpenAlexW2605754763

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.