ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2017
Recessive male sterility in cabbage (Brassica oleracea var. capitata) caused by loss of function of BoCYP704B1 due to the insertion of a LTR-retrotransposon.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- A chromosome-levelActa pharmaceutica Sinica. B · 2025Article
- Exploration of the molecular mechanism behind a novel natural genic male-sterile mutation of 1205A in Brassica napus.BMC plant biology · 2025Article
- Karyotype and LTR-RTs analysis provide insights into oak genomic evolution.BMC genomics · 2024Article
- Mutations in BrABCG26, encoding an ATP-binding cassette transporter, are responsible for male sterility in Chinese cabbage (Brassica rapa L. ssp. pekinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Map-based cloning and CRISPR/Cas9-based editing uncoverHorticulture research · 2023Article
- Genome-Wide Identification of the Cytochrome P450 Superfamily Genes and Targeted Editing ofPlants (Basel, Switzerland) · 2023Article
- BrACOS5 mutations induced male sterility via impeding pollen exine formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Mutation in BrGGL7 gene encoding a GDSL esterase / lipase causes male sterility in Chinese cabbage (Brassica rapa L. ssp. pekinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (3 Biotech · 2022Article
- Comparative Cytological and Transcriptome Analyses of Anther Development inInternational journal of molecular sciences · 2022Article
- Article
- Article
- Investigation of the genes associated with a male sterility mutant (msm) in Chinese cabbage (Brassica campestris ssp. pekinensis) using RNA-Seq.Molecular genetics and genomics : MGG · 2020Article
- Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding.Plant reproduction · 2019Review
- The anther-specific CYP704B is potentially responsible for MSG26 male sterility in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Mapping of the male sterile mutant gene ftms in Brassica rapa L. ssp. pekinensis via BSR-Seq combined with whole-genome resequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Fine mapping and candidate gene identification of the genic male-sterile gene ms3 in cabbage 51S.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
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Authors and funding
8 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
key messageThe LTR-retrotransposon insertion in BoCYP704B1 is proved to be the primary cause of the male sterility in cabbage. Effective allele-specific markers were developed for marker-assisted selection of male sterile gene. 83121A is a spontaneous male sterile mutant identified from cabbage. Genetic analysis indicated that male sterility is controlled by a single recessive gene. Pollen wall formation in the 83121A mutant was severely defective, with a lack of sporopollenin or exine. To understand the mechanisms of male sterility in 83121A, transcription analysis using RNA-Seq was carried out in the buds of the male sterile line 83121A and the male fertile line 83121B, which are near-isogenic lines differing only in the fertility trait. Via expression analysis of differentially expressed genes involved in pollen exine development before the bicellular pollen stage, BoCYP704B1 was identified as a candidate gene, which was approximately downregulated 30-fold in 83121A. BoCYP704B1 is a member of the evolutionarily conserved CYP704B family, which is essential for sporopollenin formation. The BoCYP704B1 transcript is specifically detected in the developing anthers of wild-type cabbage. Further sequence analysis revealed that a 5424-bp long terminal repeat-retrotransposon (LTR-RT) was inserted into the first exon of BoCYP704B1 in 83121A, which is not found in wild-type plants. The insertion of LTR-RT not only reduced the expression of BoCYP704B1 but also altered structure of protein encoded by BoCYP704B1. Moreover, linkage analysis showed that the homozygotic mutational BoCYP704B1 always cosegregated with male sterility. These data suggest that the LTR-RT insertion in BoCYP704B1 hinders sporopollenin formation in 83121A leading to male sterility. The allele-specific markers developed in this study were effective for marker-assisted selection of the male sterile gene.
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