Evidence map›Paper›PMID 28404944›Full record

ArticleOncotarget2017

Recombinant methioninase effectively targets a Ewing's sarcoma in a patient-derived orthotopic xenograft (PDOX) nude-mouse model.

Takashi Murakami, Shukuan Li, Qinghong Han, Yuying Tan, Tasuku Kiyuna, Kentaro Igarashi, Kei Kawaguchi, Ho Kyoung Hwang, Kentaro Miyake, Arun S Singh and 12 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
14.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 81 citations in OpenAlex.

  1. Review
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  4. Interactions between Radiation and One-Carbon Metabolism.International journal of molecular sciences · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 4 institutions in 2 countries.

Takashi MurakamiAntiCancer, Inc., San Diego, California, USA.
Shukuan LiAntiCancer, Inc., San Diego, California, USA.
Qinghong HanAntiCancer, Inc., San Diego, California, USA.
Yuying TanAntiCancer, Inc., San Diego, California, USA.
Tasuku KiyunaAntiCancer, Inc., San Diego, California, USA.
Kentaro IgarashiAntiCancer, Inc., San Diego, California, USA.
Kei KawaguchiAntiCancer, Inc., San Diego, California, USA.
Ho Kyoung HwangAntiCancer, Inc., San Diego, California, USA.
Kentaro MiyakeAntiCancer, Inc., San Diego, California, USA.
Arun S SinghDivision of Hematology-Oncology, University of California, Los Angeles, California, USA.
Scott D NelsonDepartment of Pathology, University of California, Los Angeles,California, USA.
Sarah M DryDepartment of Pathology, University of California, Los Angeles,California, USA.
Yunfeng LiDepartment of Pathology, University of California, Los Angeles,California, USA.
Yukihiko HiroshimaDepartment of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Thinzar M LwinDepartment of Surgery, University of California, San Diego, California, USA.
Jonathan C DeLongDepartment of Surgery, University of California, San Diego, California, USA.
Takashi ChishimaDepartment of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Kuniya TanakaDepartment of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Michael BouvetDepartment of Surgery, University of California, San Diego, California, USA.
Itaru EndoDepartment of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Fritz C EilberDivision of Surgical Oncology, University of California, Los Angeles, California, USA.
Robert M HoffmanAntiCancer, Inc., San Diego, California, USA.
AntiCancer (United States) · USUniversity of California, San Diego · USUniversity of California, Los Angeles · USYokohama City University · JP

Funding

Establishment of a patient-like mouse models of soft-tissue sarcoma sub-typesR43CA213649 · NCI · ANTICANCER, INC. · PI HOFFMAN, ROBERT M · 2017 to 2017
$225k
NCI NIH HHS R43 CA213649
6 · The paper itself

Abstract

Methionine dependence is due to the overuse of methionine for aberrant transmethylation reactions in cancer. Methionine dependence may be the only general metabolic defect in cancer. In order to exploit methionine dependence for therapy, our laboratory previously cloned L-methionine α-deamino-γ-mercaptomethane lyase [EC 4.4.1.11]). The cloned methioninase, termed recombinant methioninase, or rMETase, has been tested in mouse models of human cancer cell lines. Ewing's sarcoma is recalcitrant disease even though development of multimodal therapy has improved patients'outcome. Here we report efficacy of rMETase against Ewing's sarcoma in a patient-derived orthotopic xenograft (PDOX) model. The Ewing's sarcoma was implanted in the right chest wall of nude mice to establish a PDOX model. Eight Ewing's sarcoma PDOX mice were randomized into untreated control group (n = 4) and rMETase treatment group (n = 4). rMETase (100 units) was injected intraperitoneally (i.p.) every 24 hours for 14 consecutive days. All mice were sacrificed on day-15, 24 hours after the last rMETase administration. rMETase effectively reduced tumor growth compared to untreated control. The methionine level both of plasma and supernatants derived from sonicated tumors was lower in the rMETase group. Body weight did not significantly differ at any time points between the 2 groups. The present study is the first demonstrating rMETase efficacy in a PDOX model, suggesting potential clinical development, especially in recalcitrant cancers such as Ewing's sarcoma.

Indexed as

AnimalsAntimetabolites, AntineoplasticBiopsyCarbon-Sulfur LyasesDisease Models, AnimalHumansImmunohistochemistryMaleMiceMice, NudeRecombinant ProteinsSarcoma, EwingTumor BurdenXenograft Model Antitumor AssaysAntimetabolites, AntineoplasticCarbon-Sulfur LyasesL-methionine gamma-lyaseRecombinant ProteinsEwing’s sarcomamethionine dependencenude micepatient-derived orthotopic xenograftrecalcitrant cancerrecombinant methioninase

Identifiers

PMID28404944
PMCPMC5482604
OpenAlexW2593558254

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.