ArticleVeterinary research2017
Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii.
Article in Veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- Seroprevalence of Q-fever among domestic ruminants, humans and ticks in Ethiopia: A systematic review and meta-analysis.Veterinary and animal science · 2026Review
- Pathogenesis of Brucella abortus and Brucella melitensis in bovine and ovine-derived trophoblasts and macrophages and impaired intracellular trafficking of the Rev1 vaccine strain.Veterinary research · 2026Article
- Seroprevalence of Q fever among humans and animals in East Africa: a systematic review and meta-analysis.One health outlook · 2026Review
- Acid Tolerance ofPathogens (Basel, Switzerland) · 2025Article
- Navigating Q fever: Current perspectives and challenges in outbreak preparedness.Open veterinary journal · 2024Review
- Detection of Coxiella burnetii in the mammary gland of a dairy goat.Veterinary research communications · 2024Article
- Review
- Bovine blood derived macrophages are unable to controlFrontiers in immunology · 2023Article
- Phenotype ofPathogens (Basel, Switzerland) · 2022Article
- Study on the biological effects of ZnO nanosheets on EBL cells.Frontiers in bioengineering and biotechnology · 2022Article
- Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection.Virology · 2021Article
- FromPathogens (Basel, Switzerland) · 2021Review
- Bovine respiratory coronavirus enhances bacterial adherence by upregulating expression of cellular receptors on bovine respiratory epithelial cells.Veterinary microbiology · 2021Article
- Comparison ofPathogens (Basel, Switzerland) · 2020Article
- Experimental Coxiella burnetii infection in non-pregnant goats and the effect of breeding.Veterinary research · 2020Article
- Transmission ofEpidemiology and infection · 2020Article
- Defying Death - HowThe Yale journal of biology and medicine · 2019Review
- Coxiella burnetii Intratracheal Aerosol Infection Model in Mice, Guinea Pigs, and Nonhuman Primates.Infection and immunity · 2019Article
- Full-Term Human Placental Macrophages EliminateFrontiers in microbiology · 2019Article
- Development of in vitro enteroids derived from bovine small intestinal crypts.Veterinary research · 2018Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ruminants are the main source of human infections with the obligate intracellular bacterium Coxiella (C.) burnetii. Infected animals shed high numbers of C. burnetii by milk, feces, and birth products. In goats, shedding by the latter route coincides with C. burnetii replication in epithelial (trophoblast) cells of the placenta, which led us to hypothesize that epithelial cells are generally implicated in replication and shedding of C. burnetii. We therefore aimed at analyzing the interactions of C. burnetii with epithelial cells of the bovine host (1) at the entry site (lung epithelium) which govern host immune responses and (2) in epithelial cells of gut, udder and placenta decisive for the quantity of pathogen excretion. Epithelial cell lines [PS (udder), FKD-R 971 (small intestine), BCEC (maternal placenta), F3 (fetal placenta), BEL-26 (lung)] were inoculated with C. burnetii strains Nine Mile I (NMI) and NMII at different cultivation conditions. The cell lines exhibited different permissiveness for C. burnetii. While maintaining cell viability, udder cells allowed the highest replication rates with formation of large cell-filling Coxiella containing vacuoles. Intestinal cells showed an enhanced susceptibility to invasion but supported C. burnetii replication only at intermediate levels. Lung and placental cells also internalized the bacteria but in strikingly smaller numbers. In any of the epithelial cells, both Coxiella strains failed to trigger a substantial IL-1β, IL-6 and TNF-α response. Epithelial cells, with mammary epithelial cells in particular, may therefore serve as a niche for C. burnetii replication in vivo without alerting the host's immune response.
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