ArticleJournal of applied oral science : revista FOB
Human DNA extraction from whole saliva that was fresh or stored for 3, 6 or 12 months using five different protocols.
Article in Journal of applied oral science : revista FOB. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Extensive comparison of salivary collection, transportation, preparation, and storage methods: a systematic review.BMC oral health · 2024Pooled it
- Salivary RANKL and OPG gene expression quantification during intermaxillary elastic traction in orthodontic patients.MethodsX · 2026Article
- The comparison of excision, swabbing, tape-lifting, and scraping as techniques for DNA evidence recovery: A preliminary study.Forensic science international. Synergy · 2026Article
- Application properties and biosafety enhancement in saliva collection: optimization of a non-blowing medical device.BMC oral health · 2026Article
- Sex-Specific Epigenetic Patterns in Endocannabinoid System Genes Following High-Altitude Exposure: An Exploratory Study.Brain sciences · 2026Article
- Effect of Miracle Berry on Taste Modification Properties Among Adults Living in Australia: A Multi-Phase Study Protocol.Food science & nutrition · 2026Article
- Impact of face-derived DNA on touch DNA deposition on cell phone screens.International journal of legal medicine · 2026Article
- Distinct EBV-Associated Phenotypes Due to a Novel Homozygous Missense Variant inbioRxiv : the preprint server for biology · 2026Article
- Investigating oral microbiome profiles of children with cleft lip and palate in different states of caries.Journal of oral microbiology · 2026Article
- Genetic variants associated with systemic inflammatory disease associate with temporomandibular symptoms with or without periodontitis.PloS one · 2026Article
- Comparing saliva collection and DNA extraction methods for saliva-based microbiome profiling.Frontiers in microbiology · 2026Article
- Dysregulation of neurodevelopmental regulatory networks in Anorexia Nervosa: an integrated multi-layered omics analysis.Frontiers in cell and developmental biology · 2026Article
- Exploring the genetic landscape of COVID-19 susceptibility and severity among patients in Türkiye.BMC medical genomics · 2025Article
- Coordinated epigenetic dysregulation of CNR1 and FAAH genes drives endocannabinoid system dysfunction in anorexia nervosa.Journal of eating disorders · 2025Article
- Polymorphisms and environmental factors associated with orofacial clefts as potential markers for oral cancer risk.Brazilian oral research · 2025Article
- Influence of the amount of saliva deposition and time elapsed after deposition on bite mark analysis.Forensic science, medicine, and pathology · 2024Article
- Pre-Analytical Factors Affecting Extracellular DNA in Saliva.Diagnostics (Basel, Switzerland) · 2024Article
- Isolation of salivary cell-free DNA for cancer detection.PloS one · 2023Article
- Preanalytical DNA assessment for downstream applications: How to optimize the management of human biospecimens to support molecular diagnosis-An experimental study.Journal of clinical laboratory analysis · 2022Article
- Improving the Molecular Diagnosis of Malaria: Droplet Digital PCR-Based Method Using Saliva as a DNA Source.Frontiers in microbiology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
OBJECTIVE AND MATERIAL AND
methodsThis study aimed to compare the quantity and quality of human DNA extracted from saliva that was fresh or frozen for three, six and twelve months using five different DNA extraction protocols: protocol 1 - Oragene™ commercial kit, protocol 2 - QIAamp DNA mini kit, protocol 3 - DNA extraction using ammonium acetate, protocol 4 - Instagene™ Matrix and protocol 5 - Instagene™ Matrix diluted 1:1 using proteinase K and 1% SDS. Briefly, DNA was analyzed using spectrophotometry, electrophoresis and PCR.
resultsResults indicated that time spent in storage typically decreased the DNA quantity with the exception of protocol 1. The purity of DNA was generally not affected by storage times for the commercial based protocols, while the purity of the DNA samples extracted by the noncommercial protocols typically decreased when the saliva was stored longer. Only protocol 1 consistently extracted unfragmented DNA samples. In general, DNA samples extracted through protocols 1, 2, 3 and 4, regardless of storage time, were amplified by human specific primers whereas protocol 5 produced almost no samples that were able to be amplified by human specific primers. Depending on the protocol used, it was possible to extract DNA in high quantities and of good quality using whole saliva, and furthermore, for the purposes of DNA extraction, saliva can be reliably stored for relatively long time periods.
conclusionsIn summary, a complicated picture emerges when taking into account the extracted DNA's quantity, purity and quality; depending on a given researchers needs, one protocol's particular strengths and costs might be the deciding factor for its employment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.