ArticlePloS one2017
Exercise in vivo marks human myotubes in vitro: Training-induced increase in lipid metabolism.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04334343 (Investigating the Effects of Aerobic and Resistance Training in Vivo on Skeletal Muscle Metabolism in Vitro in Primary Human Muscle Cells), which is not on this map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Investigating the Effects of Aerobic and Resistance Training in Vivo on Skeletal Muscle Metabolism in Vitro in Primary Human Muscle Cells (MoTrMyo)
Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- mTORC1 signalling and protein synthesis are elevated in response to amino acids in human myotubes obtained from young, old, and old trained men.In vitro cellular & developmental biology. Animal · 2026Article
- Insights into human muscle biology from human primary skeletal muscle cell culture.Journal of muscle research and cell motility · 2025Review
- Effects of habitual endurance and resistance exercise on insulin action in primary human skeletal muscle stem cells.Physiological reports · 2025Article
- Tissue adaptation to metabolic stress: insights from SUMOylation.Frontiers in endocrinology · 2024Review
- Interplay between Cultured Human Osteoblastic and Skeletal Muscle Cells: Effects of Conditioned Media on Glucose and Fatty Acid Metabolism.Biomedicines · 2023Article
- The resting serum metabolome in response to short-term sprint interval training.European journal of applied physiology · 2023Article
- Experimental Models for Cold Exposure of MuscleBio-protocol · 2022Article
- Energy metabolism in skeletal muscle cells from donors with different body mass index.Frontiers in physiology · 2022Article
- SENP2 is vital for optimal insulin signaling and insulin-stimulated glycogen synthesis in human skeletal muscle cells.Current research in pharmacology and drug discovery · 2021Article
- Substrate oxidation in primary human skeletal muscle cells is influenced by donor age.Cell and tissue research · 2020Article
- Mitochondrial Function in Muscle Stem Cell Fates.Frontiers in cell and developmental biology · 2020Review
- Commentaries on Viewpoint: "Muscle memory" not mediated by myonuclear number? Secondary analysis of human detraining data.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- In vitro experimental models for examining the skeletal muscle cell biology of exercise: the possibilities, challenges and future developments.Pflugers Archiv : European journal of physiology · 2019Review
- Higher lipid turnover and oxidation in cultured human myotubes from athletic versus sedentary young male subjects.Scientific reports · 2018Article
- Relationship Between Sitting Time, Physical Activity, and Metabolic Syndrome Among Adults Depending on Body Mass Index (BMI).Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Lifestyle Management of Diabetes: Implications for the Bone-Vascular Axis.Current diabetes reports · 2018Review
- Functional high-intensity exercise training ameliorates insulin resistance and cardiometabolic risk factors in type 2 diabetes.Experimental physiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsPhysical activity has preventive as well as therapeutic benefits for overweight subjects. In this study we aimed to examine effects of in vivo exercise on in vitro metabolic adaptations by studying energy metabolism in cultured myotubes isolated from biopsies taken before and after 12 weeks of extensive endurance and strength training, from healthy sedentary normal weight and overweight men.
methodsHealthy sedentary men, aged 40-62 years, with normal weight (body mass index (BMI) < 25 kg/m2) or overweight (BMI ≥ 25 kg/m2) were included. Fatty acid and glucose metabolism were studied in myotubes using [14C]oleic acid and [14C]glucose, respectively. Gene and protein expressions, as well as DNA methylation were measured for selected genes.
resultsThe 12-week training intervention improved endurance, strength and insulin sensitivity in vivo, and reduced the participants' body weight. Biopsy-derived cultured human myotubes after exercise showed increased total cellular oleic acid uptake (30%), oxidation (46%) and lipid accumulation (34%), as well as increased fractional glucose oxidation (14%) compared to cultures established prior to exercise. Most of these exercise-induced increases were significant in the overweight group, whereas the normal weight group showed no change in oleic acid or glucose metabolism.
conclusions12 weeks of combined endurance and strength training promoted increased lipid and glucose metabolism in biopsy-derived cultured human myotubes, showing that training in vivo are able to induce changes in human myotubes that are discernible in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.